up Antibody

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Code	CSB-PA325666XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) up Polyclonal antibody

Uniprot No.

P19351

Target Names

Alternative Names

up antibody; int antibody; CG7107 antibody; Troponin T antibody; skeletal muscle antibody; Protein intended thorax antibody; Protein upheld antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) up protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Gene References into Functions

The present study throws light on a new role played by miR-9a during muscle development and function, showing that miR-9a is involved in the translational regulation of TnT levels during sarcomeric assembly. PMID: 28866639
Hypergravity centrifugation caused fast (i.e. within 24 h) changes to fly leg muscle TnT alternative splicing that correlated with body weight variation across eight D. melanogaster lines. Hypergravity treatment also appeared to enhance leg muscle function, as centrifuged flies showed an increased negative geotaxis response and jump ability. PMID: 28978639
Report drosophila TNT mutation causing cardiac dysfunction and remodeling comparable to that observed during human restrictive cardiomyopathy. PMID: 24221941
there are two modular enhancer elements in troponin T which establish the correct levels of Troponin T expression, adapting these levels to their specific needs PMID: 14718560
In flies overexpressing a TnT transgene, the TnT increase is seen only in early stages of adult muscle formation, followed by a decrease in this & other thin-filament proteins, maybe by triggering down-regulation of co-regulated thin-filament components. PMID: 15469415

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Protein Families

Troponin T family

Tissue Specificity

Isoform 3 is expressed in the hypoderm. Isoform 8 is expressed in the dorsal vessel. Isoform 6 is expressed in adult TDT muscle and isoform 9 in adult IFM, flight and jump muscles.

Database Links

KEGG: dme:Dmel_CG7107

STRING: 7227.FBpp0073682

UniGene: Dm.20472

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