Recombinant Drosophila melanogaster Troponin T, skeletal muscle (up)

Code CSB-YP325666DLU
MSDS
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Source Yeast
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Code CSB-EP325666DLU
MSDS
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Source E.coli
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Code CSB-EP325666DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP325666DLU
MSDS
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Source Baculovirus
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Code CSB-MP325666DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
up
Uniprot No.
Alternative Names
up; int; CG7107; Troponin T; skeletal muscle; Protein intended thorax; Protein upheld
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-397
Target Protein Sequence
MSDDEEYTSS EEEEVVEETR EETKPPQTPA EGEGDPEFIK RQDQKRSDLD DQLKEYITEW RKQRSKEEDE LKKLKEKQAK RKVTRAEEEQ KMAQRKKEEE ERRVREAEEK KQREIEEKRM RLEEAEKKRQ AMLQAMKDKD KKGPNFTIAK KDAGVLGLSS AAMERNKTKE QLEEEKKISL SFRIKPLAIE GFGEAKLREK AQELWELIVK LETEKYDLEE RQKRQDYDLK ELKERQKQQL RHKALKKGLD PEALTGKYPP KIQVASKYER RVDTRSYDDK KKLFEGGWDE ISKDSNEKIW NEKKEQYTGR QKSKLPKWFG ERPGKKAGEP ETPEGEEDAK ADEDIVEDDE EVEEEVVEEE DEEAEEDEEE EEEEEEEEEE EEEEEEEEEE EEEEEEE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. The present study throws light on a new role played by miR-9a during muscle development and function, showing that miR-9a is involved in the translational regulation of TnT levels during sarcomeric assembly. PMID: 28866639
  2. Hypergravity centrifugation caused fast (i.e. within 24 h) changes to fly leg muscle TnT alternative splicing that correlated with body weight variation across eight D. melanogaster lines. Hypergravity treatment also appeared to enhance leg muscle function, as centrifuged flies showed an increased negative geotaxis response and jump ability. PMID: 28978639
  3. Report drosophila TNT mutation causing cardiac dysfunction and remodeling comparable to that observed during human restrictive cardiomyopathy. PMID: 24221941
  4. there are two modular enhancer elements in troponin T which establish the correct levels of Troponin T expression, adapting these levels to their specific needs PMID: 14718560
  5. In flies overexpressing a TnT transgene, the TnT increase is seen only in early stages of adult muscle formation, followed by a decrease in this & other thin-filament proteins, maybe by triggering down-regulation of co-regulated thin-filament components. PMID: 15469415

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Protein Families
Troponin T family
Tissue Specificity
Isoform 3 is expressed in the hypoderm. Isoform 8 is expressed in the dorsal vessel. Isoform 6 is expressed in adult TDT muscle and isoform 9 in adult IFM, flight and jump muscles.
Database Links

KEGG: dme:Dmel_CG7107

STRING: 7227.FBpp0073682

UniGene: Dm.20472

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