tnrA Antibody

Code CSB-PA670457XA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) tnrA Polyclonal antibody
Uniprot No.
Target Names
tnrA
Alternative Names
tnrA antibody; scgR antibody; BSU13310 antibody; HTH-type transcriptional regulator TnrA antibody; Transcriptional nitrogen regulatory protein A antibody; TnrA antibody
Raised in
Rabbit
Species Reactivity
Bacillus subtilis (strain 168)
Immunogen
Recombinant Bacillus subtilis (strain 168) tnrA protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription regulator that actives the transcription of genes required for nitrogen assimilation such as nrgAB (ammonium transport), nasABCDEF (nitrate/nitrite assimilation), ureABC (urea degradation) and gabP (GABA transport), during nitrogen limitation. Also represses glnRA and gltAB in the absence of ammonium. On the contrary of the MerR members, which require longer DNA sites for high-affinity binding, TnrA requires a DNA sequence of 17 nucleotides as minimal binding site.
Gene References into Functions
  1. Data suggest GS (glutamine synthetase) controls TnrA activity at positively controlled promoters by stabilizing TnrA in DNA-bound state; ATP and glutamine bind to GS in competitive manner; AMP competes with ATP and favors GS--TnrA interaction. PMID: 26635369
  2. TnrA inhibits the biosynthetic activity of glutamine synthetase PMID: 23535029
  3. TnrA binding to GlnK or glutamine synthetase responds differentially to adenylate nucleotide levels, with ATP weakening interactions with both partners. PMID: 21435182
  4. The negative control of the expression of the ilv-leu operon by TnrA, which is considered to represent rather fine-tuning (two- to threefold), is a novel regulatory link between nitrogen and amino acid metabolism. PMID: 15547269
  5. The biochemical properties of NrgB and its interactions with NrgA and TnrA are reported. PMID: 17001076
  6. TnrA transcription factor regulates gene expression during nitrogen-limited growth. PMID: 17085574
  7. The proteolytic degradation of TnrA as a response to nitrogen starvation represent a novel mechanism of TnrA control in B. subtilis. PMID: 18667567

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Subcellular Location
Cell membrane.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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