Recombinant Bacillus subtilis HTH-type transcriptional regulator tnrA (tnrA)

Code CSB-YP670457BRJ
MSDS
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Source Yeast
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Code CSB-EP670457BRJ
MSDS
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Source E.coli
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Code CSB-EP670457BRJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP670457BRJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP670457BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tnrA
Uniprot No.
Alternative Names
tnrA; scgR; BSU13310; HTH-type transcriptional regulator TnrA; Transcriptional nitrogen regulatory protein A; TnrA
Species
Bacillus subtilis (strain 168)
Expression Region
1-110
Target Protein Sequence
MTTEDHSYKD KKVISIGIVS ELTGLSVRQI RYYEERKLIY PQRSSRGTRK YSFADVERLM DIANKREDGV QTAEILKDMR KKEQMLKNDP QVRKKMLEGQ LNAHFRYKNR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription regulator that actives the transcription of genes required for nitrogen assimilation such as nrgAB (ammonium transport), nasABCDEF (nitrate/nitrite assimilation), ureABC (urea degradation) and gabP (GABA transport), during nitrogen limitation. Also represses glnRA and gltAB in the absence of ammonium. On the contrary of the MerR members, which require longer DNA sites for high-affinity binding, TnrA requires a DNA sequence of 17 nucleotides as minimal binding site.
Gene References into Functions
  1. Data suggest GS (glutamine synthetase) controls TnrA activity at positively controlled promoters by stabilizing TnrA in DNA-bound state; ATP and glutamine bind to GS in competitive manner; AMP competes with ATP and favors GS--TnrA interaction. PMID: 26635369
  2. TnrA inhibits the biosynthetic activity of glutamine synthetase PMID: 23535029
  3. TnrA binding to GlnK or glutamine synthetase responds differentially to adenylate nucleotide levels, with ATP weakening interactions with both partners. PMID: 21435182
  4. The negative control of the expression of the ilv-leu operon by TnrA, which is considered to represent rather fine-tuning (two- to threefold), is a novel regulatory link between nitrogen and amino acid metabolism. PMID: 15547269
  5. The biochemical properties of NrgB and its interactions with NrgA and TnrA are reported. PMID: 17001076
  6. TnrA transcription factor regulates gene expression during nitrogen-limited growth. PMID: 17085574
  7. The proteolytic degradation of TnrA as a response to nitrogen starvation represent a novel mechanism of TnrA control in B. subtilis. PMID: 18667567

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Subcellular Location
Cell membrane.
Database Links
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