Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TOC33 Polyclonal antibody

Uniprot No.

O23680

Target Names

TOC33

Alternative Names

TOC33; PPI1; At1g02280; T7I23.11; Translocase of chloroplast 33, chloroplastic; AtToc33; 33 kDa chloroplast outer envelope protein; Plastid protein import 1

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TOC33 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Binds GTP, GDP, XTP, but not ATP. Probably specialized in the import of nuclear encoded photosynthetic preproteins from the cytoplasm to the chloroplast, especially during early development stages.

Gene References into Functions

[review] Homodimerization of Toc33 prevents nucleotide exchange, thereby locking the receptor in the guanosine diphosphate (GDP)-loaded state and preventing further activity. PMID: 21568939
Pre-protein binding induces a dissociation of the Toc33 homodimer and results in exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). PMID: 21434866
Neither nucleotide binding nor dimerization at atToc33 is essential for chloroplast import. PMID: 20444229
The role of Toc33 protein in biological transport of proteins in the protochlorophyllide-dependent pathway in A. thaliana. PMID: 15773849
The data suggest that atToc33 phosphorylation may not play an important role in vivo. PMID: 16412428
Chloroplast biogenesis and photosynthetic activity are impaired in the mutants during the early developmental stage, which is consistent with the requirement of atToc33 in young photosynthetic tissues. PMID: 18054337
Dimerization is independent of nucleotide loading state, based on crystal structures of dimeric Pisum sativum Toc34 and monomeric Arabidopsis thaliana Toc33. PMID: 18400179
Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive. PMID: 19001421

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Subcellular Location

Plastid, chloroplast outer membrane; Single-pass membrane protein. Note=May contain beta barrel transmembrane regions.

Protein Families

TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, TOC34 subfamily

Tissue Specificity

Mostly expressed in seedlings and flowers, and, to a lower extent, in roots, stems, and leaves.

Database Links

KEGG: ath:AT1G02280

STRING: 3702.AT1G02280.1

UniGene: At.10710

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TOC33 Antibody

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