TPI1 Antibody

Code CSB-PA024102XA01CH
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Product Details

Full Product Name
Rabbit anti-Gallus gallus (Chicken) TPI1 Polyclonal antibody
Uniprot No.
Target Names
TPI1
Alternative Names
TPI1 antibody; Triosephosphate isomerase antibody; TIM antibody; EC 5.3.1.1 antibody; Methylglyoxal synthase antibody; EC 4.2.3.3 antibody; Triose-phosphate isomerase antibody
Raised in
Rabbit
Species Reactivity
Gallus gallus (Chicken)
Immunogen
Recombinant Gallus gallus (Chicken) TPI1 protein
Immunogen Species
Gallus gallus (Chicken)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.; It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
Gene References into Functions
  1. The hypothesis that limited access to conformational space is a requirement for protein hinges involved in catalysis has been successfully tested with glycine N- and C-terminal hinge mutants of loop 6 of triosephosphate isomerase (TIM). PMID: 15350130
  2. NMR study of wild-type (WT) TIM and a quintuple mutant (PGG/GGG). PMID: 17336327
  3. There is clear evidence for motion with an exchange rate constant consistent with opening and closing of triosephosphate isomerase active site loop (loop 6) being partially rate-limiting to catalytic throughput. PMID: 17455914
  4. 1st essential mode contributing to 34% of overall motion of dimer is strongly coupled to loop 6's closure over active site. Onset of collective motions at ns time scale due to dimerization has functional implications as to coordination of loop 6 closure. PMID: 18189421
Subcellular Location
Cytoplasm.
Protein Families
Triosephosphate isomerase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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