tra2 Antibody

Code CSB-PA323071XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) tra2 Polyclonal antibody
Uniprot No.
Target Names
tra2
Alternative Names
tra2 antibody; tra-2 antibody; CG10128 antibody; Transformer-2 sex-determining protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) tra2 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for female sex determination in somatic cells and for spermatogenesis in male germ cells. Positive regulator of female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA. Splicing requires an enhancer complex, dsxRE (dsx repeat element: which contains six copies of a 13-nucleotide repeat and a purine-rich enhancer (PRE)). DsxRE is formed through cooperative interactions between tra, tra2 and the sr proteins, and these interactions require both the repeat sequences and PRE. PRE is required for specific binding of tra2 to the dsxRE. Protein-RNA and protein-protein interactions are involved in tra-2 dependent activation and repression of alternative splicing. Together with tra-2, plays a role in switching fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site.
Gene References into Functions
  1. Together, these results suggest a link between mRNA splicing, sex determination and lipid metabolism and may provide insight into the mechanisms underlying tissue-specific splicing and nutrient storage. PMID: 29203241
  2. results indicate the Hfp/Puf68 protein is required for the negative regulation of splicing by Tra2 PMID: 23880637
  3. Anastrepha Tra2 protein supplies tra-2 function in Drosophila PMID: 22252495
  4. Tra2 represses M1 splicing by interacting with multiple sequences in the pre-mRNA and interfering with enhancer function PMID: 12861004
  5. Here we show that that widely used tra2 mutant alleles have residual activity and are not suitable for evaluating its effect on viability. PMID: 14595843
  6. Rbp1 and Tra2 bind to the ISS independently through distinct sequences suggesting that functionally synergistic interactions of these SR factors can cause either splicing activation or repression. PMID: 17101798

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Protein Families
Splicing factor SR family
Tissue Specificity
Isoform Tmaj and isoform Tmin are expressed in males and females. Isoform msTmaj and isoform msTmin are present only in male germ cells.
Database Links

KEGG: dme:Dmel_CG10128

STRING: 7227.FBpp0088562

UniGene: Dm.4216

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