Recombinant Drosophila melanogaster Transformer-2 sex-determining protein (tra2)

Code CSB-YP323071DLU
MSDS
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Source Yeast
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Code CSB-EP323071DLU
MSDS
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Source E.coli
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Code CSB-EP323071DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323071DLU
MSDS
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Source Baculovirus
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Code CSB-MP323071DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tra2
Uniprot No.
Alternative Names
tra2; tra-2; CG10128; Transformer-2 sex-determining protein
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-264
Target Protein Sequence
MDREPLSSGR LHCSARYKHK RSASSSSAGT TSSGHKDRRS DYDYCGSRRH QRSSSRRRSR SRSSSESPPP EPRHRSGRSS RDRERMHKSR EHPQASRCIG VFGLNTNTSQ HKVRELFNKY GPIERIQMVI DAQTQRSRGF CFIYFEKLSD ARAAKDSCSG IEVDGRRIRV DFSITQRAHT PTPGVYLGRQ PRGKAPRSFS PRRGRRVYHD RSASPYDNYR DRYDYRNDRY DRNLRRSPSR NRYTRNRSYS RSRSPQLRRT SSRY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for female sex determination in somatic cells and for spermatogenesis in male germ cells. Positive regulator of female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA. Splicing requires an enhancer complex, dsxRE (dsx repeat element: which contains six copies of a 13-nucleotide repeat and a purine-rich enhancer (PRE)). DsxRE is formed through cooperative interactions between tra, tra2 and the sr proteins, and these interactions require both the repeat sequences and PRE. PRE is required for specific binding of tra2 to the dsxRE. Protein-RNA and protein-protein interactions are involved in tra-2 dependent activation and repression of alternative splicing. Together with tra-2, plays a role in switching fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site.
Gene References into Functions
  1. Together, these results suggest a link between mRNA splicing, sex determination and lipid metabolism and may provide insight into the mechanisms underlying tissue-specific splicing and nutrient storage. PMID: 29203241
  2. results indicate the Hfp/Puf68 protein is required for the negative regulation of splicing by Tra2 PMID: 23880637
  3. Anastrepha Tra2 protein supplies tra-2 function in Drosophila PMID: 22252495
  4. Tra2 represses M1 splicing by interacting with multiple sequences in the pre-mRNA and interfering with enhancer function PMID: 12861004
  5. Here we show that that widely used tra2 mutant alleles have residual activity and are not suitable for evaluating its effect on viability. PMID: 14595843
  6. Rbp1 and Tra2 bind to the ISS independently through distinct sequences suggesting that functionally synergistic interactions of these SR factors can cause either splicing activation or repression. PMID: 17101798

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Protein Families
Splicing factor SR family
Tissue Specificity
Isoform Tmaj and isoform Tmin are expressed in males and females. Isoform msTmaj and isoform msTmin are present only in male germ cells.
Database Links

KEGG: dme:Dmel_CG10128

STRING: 7227.FBpp0088562

UniGene: Dm.4216

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