TRMT5 Antibody

Code CSB-PA656288XA01HU
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TRMT5 Polyclonal antibody
Uniprot No.
Target Names
TRMT5
Alternative Names
TRMT5 antibody; KIAA1393 antibody; TRM5 antibody; tRNA antibody; guanine(37)-N1)-methyltransferase antibody; EC 2.1.1.228 antibody; M1G-methyltransferase antibody; tRNA [GM37] methyltransferase antibody; tRNA methyltransferase 5 homolog antibody
Raised in
Rabbit
Species Reactivity
Homo sapiens (Human)
Immunogen
Recombinant Homo sapiens (Human) TRMT5 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in mitochondrial tRNA methylation. Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Gene References into Functions
  1. TRMT5 mutations cause a defect in post-transcriptional modification of mitochondrial tRNA associated with multiple respiratory-chain deficiencies. PMID: 26189817
  2. Conservation of structure and mechanism by Trm5 enzymes PMID: 23887145
  3. comparison of human TRM5 and E Coli TrmD activities, requirements for optimal activity, and tRNA methylation sites PMID: 15248782
Involvement in disease
Combined oxidative phosphorylation deficiency 26 (COXPD26)
Subcellular Location
Mitochondrion matrix. Nucleus. Cytoplasm.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, TRM5/TYW2 family
Database Links

HGNC: 23141

OMIM: 611023

KEGG: hsa:57570

STRING: 9606.ENSP00000261249

UniGene: Hs.380159

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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