trr Antibody

Code CSB-PA818221XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) trr Polyclonal antibody
Uniprot No.
Target Names
trr
Alternative Names
trr antibody; KMT2C antibody; CG3848Histone-lysine N-methyltransferase trr antibody; EC 2.1.1.354 antibody; Lysine N-methyltransferase 2C antibody; Trithorax-related protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) trr protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone methyltransferase that acts as a coactivator for the ecdysone receptor during development. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation. Recruited by EcR in an ecdysone-dependent manner causing H3 'Lys-4' trimethylation at ecdysone-inducible promoters, leading to activate expression. Plays a central role in the developing compound eye, during the progression of the morphogenetic furrow and in post-furrow differentiation of the retinal epithelium, notably by activating expression of hh. Also required for wing and abdominal development.
Gene References into Functions
  1. Histone H3K4 monomethylation catalyzed by Trr and mammalian MML3/MLL4 proteins at enhancers is dispensable for development and viability. PMID: 28967912
  2. Ash2 functions together with Trr as an ecdysone receptor coactivator. PMID: 23197473
  3. trr mutant cells have a growth advantage over their wild-type neighbors and display changes in the levels of multiple proteins that regulate growth and cell division, including Notch, Capicua, and cyclin B. PMID: 23459941
  4. Trr, the Drosophila homolog of the mammalian Mll3/4 COMPASS-like complexes, can function as a major H3K4 monomethyltransferase on enhancers in vivo. PMID: 23166019
  5. TRR is a histone methyltransferases capable of trimethylating lysine 4 of histone H3 (H3-K4); TRR possibly functions as a coactivator of ecdysone receptor by altering the chromatin structure at ecdysone-responsive promoters PMID: 14603321

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, TRX/MLL subfamily
Tissue Specificity
Widely expressed.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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