tra Antibody

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Code	CSB-PA319317XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) tra Polyclonal antibody

Uniprot No.

P11596

Target Names

tra

Alternative Names

tra antibody; CG16724 antibody; Female-specific protein transformer antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) tra protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Member of the regulatory pathway controlling female somatic sexual differentiation, regulated by Sxl. Activates dsx female-specific splicing by promoting the formation of a splicing enhancer complex which consists of tra, tra2 and sr proteins. Together with tra-2, plays a role in switching fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site. No known function in males.

Gene References into Functions

Transcriptional control of the sex determination hierarchy by Chinmo is critical for sex maintenance in sexually dimorphic tissues and is vital in the preservation of fertility. Chinmo prevents transformer alternative splicing to maintain male sex identity. PMID: 29389999
Drosophila female-specific Ilp7 motoneurons are generated by Fruitless-dependent cell death in males and by a double-assurance survival role for Transformer in females PMID: 29229771
tra expression play crucial role in determining sex-specific size in Drosophila larvae and adult tissue. PMID: 28064166
body size is regulated by tra in a sex-specific fashion. PMID: 26710087
When tsh(GAL4) drove expression of transformer (tra), males were unable to produce prolonged wing extensions. PMID: 20919857
Effect of tra of Tephritidae on the somatic sexual development of Drosophila are reported. PMID: 20209435
tra sequence variation among members of the Drosophila melanogaster species complex, D. melanogaster, D. simulans, D. mauritiana, and D. sechellia PMID: 12644565
In gynanders (XX//XO mosaics) feminized by this Tra(F) transgene, functionally Sxl- haplo-X (chromosomally male) somatic cells collaborated successfully with diplo-X (chromosomally female) germ cells to make functional eggs. PMID: 17110478
the reciprocal regulatory effect by studying genetically sensitized females: TRA-F from either maternal or zygotic tra expression stimulates sex-lethal -positive autoregulation. PMID: 18845845

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Subcellular Location

Nucleus speckle. Note=Speckled subnuclear compartment.

Database Links

KEGG: dme:Dmel_CG16724

STRING: 7227.FBpp0075123

UniGene: Dm.29698

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