Recombinant Drosophila melanogaster Female-specific protein transformer (tra)

Code CSB-YP319317DLU
MSDS
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Source Yeast
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Code CSB-EP319317DLU
MSDS
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Source E.coli
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Code CSB-EP319317DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP319317DLU
MSDS
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Source Baculovirus
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Code CSB-MP319317DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tra
Uniprot No.
Alternative Names
tra; CG16724; Female-specific protein transformer
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-197
Target Protein Sequence
MKMDADSSGT QHRDSRGSRS RSRREREYHG RSSERDSRKK EHKIPYFADE VREQDRLRRL RQRAHQSTRR TRSRSRSQSS IRESRHRRHR QRSRSRNRSR SRSSERKRRQ RSRSRSSERR RRQRSPHRYN PPPKIINYYV QVPPQDFYGM SGMQQSFGYQ RLPRPPPFPP APYRYRQRPP FIGVPRFGYR NAGRPPY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Member of the regulatory pathway controlling female somatic sexual differentiation, regulated by Sxl. Activates dsx female-specific splicing by promoting the formation of a splicing enhancer complex which consists of tra, tra2 and sr proteins. Together with tra-2, plays a role in switching fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site. No known function in males.
Gene References into Functions
  1. Transcriptional control of the sex determination hierarchy by Chinmo is critical for sex maintenance in sexually dimorphic tissues and is vital in the preservation of fertility. Chinmo prevents transformer alternative splicing to maintain male sex identity. PMID: 29389999
  2. Drosophila female-specific Ilp7 motoneurons are generated by Fruitless-dependent cell death in males and by a double-assurance survival role for Transformer in females PMID: 29229771
  3. tra expression play crucial role in determining sex-specific size in Drosophila larvae and adult tissue. PMID: 28064166
  4. body size is regulated by tra in a sex-specific fashion. PMID: 26710087
  5. When tsh(GAL4) drove expression of transformer (tra), males were unable to produce prolonged wing extensions. PMID: 20919857
  6. Effect of tra of Tephritidae on the somatic sexual development of Drosophila are reported. PMID: 20209435
  7. tra sequence variation among members of the Drosophila melanogaster species complex, D. melanogaster, D. simulans, D. mauritiana, and D. sechellia PMID: 12644565
  8. In gynanders (XX//XO mosaics) feminized by this Tra(F) transgene, functionally Sxl- haplo-X (chromosomally male) somatic cells collaborated successfully with diplo-X (chromosomally female) germ cells to make functional eggs. PMID: 17110478
  9. the reciprocal regulatory effect by studying genetically sensitized females: TRA-F from either maternal or zygotic tra expression stimulates sex-lethal -positive autoregulation. PMID: 18845845

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Subcellular Location
Nucleus speckle. Note=Speckled subnuclear compartment.
Database Links
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