TT8 Antibody

Code CSB-PA884326XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TT8 Polyclonal antibody
Uniprot No.
Target Names
TT8
Alternative Names
TT8 antibody; BHLH42 antibody; EN32 antibody; At4g09820 antibody; F17A8.170 antibody; Transcription factor TT8 antibody; Basic helix-loop-helix protein 42 antibody; AtbHLH42 antibody; bHLH 42 antibody; Protein TRANSPARENT TESTA 8 antibody; Transcription factor EN 32 antibody; bHLH transcription factor bHLH042 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TT8 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator, when associated with MYB75/PAP1 or MYB90/PAP2. Involved in the control of flavonoid pigmentation. Plays a key role in regulating leucoanthocyanidin reductase (BANYULS) and dihydroflavonol-4-reductase (DFR). Not required for leucoanthocyanidin dioxygenase (LDOX) expression.
Gene References into Functions
  1. the C-terminal domain of the bHLH members (GLABRA3 [GL3], ENHANCER OF GLABRA3 [EGL3] and TRANSPARENT TESTA8 [TT8]) interacted with JAZs. PMID: 29293407
  2. The transcription factor TT8 is an integrator of secondary metabolism and stress response. PMID: 27432888
  3. Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26Sproteasome system PMID: 23921543
  4. These results provide a molecular model consistent with the specific and highly regulated expression of TT8. PMID: 23398515
  5. Nitrogen regulation of anthocyanin biosynthesis in Arabidopsis thaliana red cells undergoes a mechanism by which nitrogen controls the expression of genes encoding both main components of the TTG1-GL3/TT8-PAP1 complex and negative regulators. PMID: 22669605
  6. TT8 expression is controlled by different combinations of MYB and bHLH factors in planta. PMID: 16709193

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Subcellular Location
Nucleus.
Protein Families
BHLH protein family
Tissue Specificity
Buds, flowers and developing siliques, but not in leaves, stems and roots.
Database Links

KEGG: ath:AT4G09820

STRING: 3702.AT4G09820.1

UniGene: At.10175

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