Recombinant Arabidopsis thaliana Transcription factor TT8 (TT8), partial

Code CSB-YP884326DOA
MSDS
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Source Yeast
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Code CSB-EP884326DOA
MSDS
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Source E.coli
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Code CSB-EP884326DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884326DOA
MSDS
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Source Baculovirus
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Code CSB-MP884326DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TT8
Uniprot No.
Alternative Names
TT8; BHLH42; EN32; At4g09820; F17A8.170; Transcription factor TT8; Basic helix-loop-helix protein 42; AtbHLH42; bHLH 42; Protein TRANSPARENT TESTA 8; Transcription factor EN 32; bHLH transcription factor bHLH042
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator, when associated with MYB75/PAP1 or MYB90/PAP2. Involved in the control of flavonoid pigmentation. Plays a key role in regulating leucoanthocyanidin reductase (BANYULS) and dihydroflavonol-4-reductase (DFR). Not required for leucoanthocyanidin dioxygenase (LDOX) expression.
Gene References into Functions
  1. the C-terminal domain of the bHLH members (GLABRA3 [GL3], ENHANCER OF GLABRA3 [EGL3] and TRANSPARENT TESTA8 [TT8]) interacted with JAZs. PMID: 29293407
  2. The transcription factor TT8 is an integrator of secondary metabolism and stress response. PMID: 27432888
  3. Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26Sproteasome system PMID: 23921543
  4. These results provide a molecular model consistent with the specific and highly regulated expression of TT8. PMID: 23398515
  5. Nitrogen regulation of anthocyanin biosynthesis in Arabidopsis thaliana red cells undergoes a mechanism by which nitrogen controls the expression of genes encoding both main components of the TTG1-GL3/TT8-PAP1 complex and negative regulators. PMID: 22669605
  6. TT8 expression is controlled by different combinations of MYB and bHLH factors in planta. PMID: 16709193

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Subcellular Location
Nucleus.
Protein Families
BHLH protein family
Tissue Specificity
Buds, flowers and developing siliques, but not in leaves, stems and roots.
Database Links

KEGG: ath:AT4G09820

STRING: 3702.AT4G09820.1

UniGene: At.10175

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