ECH Antibody

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Code	CSB-PA847512XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ECH Polyclonal antibody

Uniprot No.

Q8LEK2

Target Names

ECH

Alternative Names

ECH; At1g09330; T31J12.5; Golgi apparatus membrane protein-like protein ECHIDNA

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ECH protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Mediates trans-Golgi-network trafficking and cell elongation. Required for keeping the appropriate balance between secretory trafficking and vacuolar targeting of a subset of proteins. The ECH/YIP4 complex is involved in the modulation of the trans-Golgi network (TGN)-mediated trafficking of some proteins and cell wall components (e.g. pectin and hemicellulose) to the cell wall in dark-grown hypocotyls and in secretory cells of the seed coat.

Gene References into Functions

ECH expression is critical for a variety of aspects of male reproduction, including the production of functional pollen grains, their effective release, germination, and tube formation. PMID: 24424320
ECH protein is required for the trans-Golgi network-mediated trafficking of the auxin influx carrier AUX1 to the plasma membrane. [ECH] PMID: 24043780

Subcellular Location

Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein.

Protein Families

TVP23 family

Database Links

KEGG: ath:AT1G09330

STRING: 3702.AT1G09330.1

UniGene: At.43661

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ECH Antibody

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