Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TDP2 Polyclonal antibody

Uniprot No.

O95551

Target Names

TDP2

Alternative Names

5'-Tyr-DNA phosphodiesterase antibody; 5'-tyrosyl-DNA phosphodiesterase antibody; AD 022 antibody; AD022 antibody; dJ30M3.3 antibody; EAP 2 antibody; EAP II antibody; EAP2 antibody; EAPII antibody; ETS 1 associated protein 2 antibody; ETS 1 associated protein II antibody; ETS1 associated protein 2 antibody; ETS1 associated protein II antibody; ETS1-associated protein 2 antibody; ETS1-associated protein II antibody; MGC111021 antibody; MGC9099 antibody; RP1-30M3.3 antibody; tdp2 antibody; TRAF and TNF receptor associated protein antibody; TRAF and TNF receptor-associated protein antibody; TTRAP antibody; TYDP2_HUMAN antibody; Tyr DNA phosphodiesterase 2 antibody; Tyr-DNA phosphodiesterase 2 antibody; Tyrosyl DNA phosphodiesterase 2 antibody; Tyrosyl-DNA phosphodiesterase 2 antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) TDP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation.; (Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA.

Gene References into Functions

Data indicate that tyrosyl-DNA phosphodiesterase 2 (TDP2) alone does not remove DNA topoisomerase II (TOP2)-DNA complexes from genomic DNA in vitro and that depletion of TDP2 in cells does not slow the removal of TOP2-DNA complexes. PMID: 30011940
TDP2 re-localizes from the nucleus to the cytoplasm of EMCV infected cells. PMID: 29353210
TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription. PMID: 28794467
Both the genome instability and cell death of MRE11-null and MRE11-mutated H129N cells are significantly reversed by overexpression of Tdp2, an enzyme that eliminates covalent Top2 conjugates; thus, the essential role of Mre11 nuclease activity is likely to remove the DNA lesions. PMID: 27814490
Study revealed a post-translational regulation of TDP2 activity and discovered a new role of ERK3 in increasing cancer cells' DNA damage response and chemoresistance to Top2 inhibitors. PMID: 26701725
Data show that DNA-repair enzyme TDP2 knockdown in HepG2 cells significantly delays the conversion of relaxed circular DNA (RC-DNA) to covalently closed circular (ccc) DNA. PMID: 25201958
This article summarizes and compares the biochemistry, functions, and post-translational regulation of TDP1 and TDP2, as well as the relevance of TDP1 and TDP2 as determinants of response to anticancer agents PMID: 24856239
Proteolytic degradation of Top2 enables the processing of Top2.DNA and Top2.RNA covalent complexes by TDP2. PMID: 24808172
TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function. PMID: 24658003
Mutations in the 5'-tyrosyl-DNA phosphodiesterase activity of TTRAP,in particular TTRAP(E152A), showed decreased inhibitory activity on cell growth. PMID: 23433115
Magnesium is indispensable for a 5'-tyrosyl DNA phosphodiesterase activity. PMID: 22405347
VPg unlinkase activity in different mammalian cell lines correlates with their differential expression of TDP2. PMID: 22908287
Biochemical characterization of human tyrosyl-DNA phosphodiesterase 2 (TDP2/TTRAP): a Mg(2+)/Mn(2+)-dependent phosphodiesterase specific for the repair of topoisomerase cleavage complexes. PMID: 22822062
findings suggest a previously unidentified function for TTRAP and nucleolar cavities in ribosome biogenesis under stress. PMID: 21921940
both FOXP2 and KIAA0319/TTRAP/THEM2 genes play an important role in human language development, but probably through different cerebral pathways. PMID: 22262880
EAPII is an oncogenic factor and the activation of MAPK-ERK signaling pathway by EAPII may contribute to lung cancer development. PMID: 21478903
an important mechanism for resistance to Top2-induced chromosome breakage and the possibility that TDP2 is a significant factor in cancer development and treatment PMID: 21030584
PhiC31 integrase interacts with TTRAP and inhibits the NFkappaB activation. PMID: 19757154
interacts with ETS1 and modulates its transcriptional function PMID: 12743594
a significant association with (TTRAP)on reading and spelling ability was observed PMID: 17597587
TTRAP is a novel PML nuclear body protein. PMID: 18706885
This is the first time that TTRAP has been shown to interact with HIV-1 integrase and facilitate lentiviral vector integration. PMID: 19580783
TTRAP is the first human 5'-tyrosyl DNA phosphodiesterase to be identified, and we suggest that this enzyme is denoted tyrosyl DNA phosphodiesterase-2 (TDP2). PMID: 19794497

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Involvement in disease

Spinocerebellar ataxia, autosomal recessive, 23 (SCAR23)

Subcellular Location

Nucleus. Nucleus, PML body. Nucleus, nucleolus. Cytoplasm.; Cytoplasm.

Protein Families

CCR4/nocturin family

Tissue Specificity

Widely expressed. Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum.

Database Links

HGNC: 17768

OMIM: 605764

KEGG: hsa:51567

STRING: 9606.ENSP00000367440

UniGene: Hs.403010

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TDP2 Antibody

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