Product Details

Uniprot No.

Target Names

TDP2

Alternative Names

5'-Tyr-DNA phosphodiesterase antibody; 5'-tyrosyl-DNA phosphodiesterase antibody; AD 022 antibody; AD022 antibody; dJ30M3.3 antibody; EAP 2 antibody; EAP II antibody; EAP2 antibody; EAPII antibody; ETS 1 associated protein 2 antibody; ETS 1 associated protein II antibody; ETS1 associated protein 2 antibody; ETS1 associated protein II antibody; ETS1-associated protein 2 antibody; ETS1-associated protein II antibody; MGC111021 antibody; MGC9099 antibody; RP1-30M3.3 antibody; tdp2 antibody; TRAF and TNF receptor associated protein antibody; TRAF and TNF receptor-associated protein antibody; TTRAP antibody; TYDP2_HUMAN antibody; Tyr DNA phosphodiesterase 2 antibody; Tyr-DNA phosphodiesterase 2 antibody; Tyrosyl DNA phosphodiesterase 2 antibody; Tyrosyl-DNA phosphodiesterase 2 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide of Human TDP2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation.; (Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA.

Gene References into Functions

Data indicate that tyrosyl-DNA phosphodiesterase 2 (TDP2) alone does not remove DNA topoisomerase II (TOP2)-DNA complexes from genomic DNA in vitro and that depletion of TDP2 in cells does not slow the removal of TOP2-DNA complexes. PMID: 30011940
TDP2 re-localizes from the nucleus to the cytoplasm of EMCV infected cells. PMID: 29353210
TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription. PMID: 28794467
Both the genome instability and cell death of MRE11-null and MRE11-mutated H129N cells are significantly reversed by overexpression of Tdp2, an enzyme that eliminates covalent Top2 conjugates; thus, the essential role of Mre11 nuclease activity is likely to remove the DNA lesions. PMID: 27814490
Study revealed a post-translational regulation of TDP2 activity and discovered a new role of ERK3 in increasing cancer cells' DNA damage response and chemoresistance to Top2 inhibitors. PMID: 26701725
Data show that DNA-repair enzyme TDP2 knockdown in HepG2 cells significantly delays the conversion of relaxed circular DNA (RC-DNA) to covalently closed circular (ccc) DNA. PMID: 25201958
This article summarizes and compares the biochemistry, functions, and post-translational regulation of TDP1 and TDP2, as well as the relevance of TDP1 and TDP2 as determinants of response to anticancer agents PMID: 24856239
Proteolytic degradation of Top2 enables the processing of Top2.DNA and Top2.RNA covalent complexes by TDP2. PMID: 24808172
TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function. PMID: 24658003
Mutations in the 5'-tyrosyl-DNA phosphodiesterase activity of TTRAP,in particular TTRAP(E152A), showed decreased inhibitory activity on cell growth. PMID: 23433115
Magnesium is indispensable for a 5'-tyrosyl DNA phosphodiesterase activity. PMID: 22405347
VPg unlinkase activity in different mammalian cell lines correlates with their differential expression of TDP2. PMID: 22908287
Biochemical characterization of human tyrosyl-DNA phosphodiesterase 2 (TDP2/TTRAP): a Mg(2+)/Mn(2+)-dependent phosphodiesterase specific for the repair of topoisomerase cleavage complexes. PMID: 22822062
findings suggest a previously unidentified function for TTRAP and nucleolar cavities in ribosome biogenesis under stress. PMID: 21921940
both FOXP2 and KIAA0319/TTRAP/THEM2 genes play an important role in human language development, but probably through different cerebral pathways. PMID: 22262880
EAPII is an oncogenic factor and the activation of MAPK-ERK signaling pathway by EAPII may contribute to lung cancer development. PMID: 21478903
an important mechanism for resistance to Top2-induced chromosome breakage and the possibility that TDP2 is a significant factor in cancer development and treatment PMID: 21030584
PhiC31 integrase interacts with TTRAP and inhibits the NFkappaB activation. PMID: 19757154
interacts with ETS1 and modulates its transcriptional function PMID: 12743594
a significant association with (TTRAP)on reading and spelling ability was observed PMID: 17597587
TTRAP is a novel PML nuclear body protein. PMID: 18706885
This is the first time that TTRAP has been shown to interact with HIV-1 integrase and facilitate lentiviral vector integration. PMID: 19580783
TTRAP is the first human 5'-tyrosyl DNA phosphodiesterase to be identified, and we suggest that this enzyme is denoted tyrosyl DNA phosphodiesterase-2 (TDP2). PMID: 19794497

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Involvement in disease

Spinocerebellar ataxia, autosomal recessive, 23 (SCAR23)

Subcellular Location

Nucleus. Nucleus, PML body. Nucleus, nucleolus. Cytoplasm.; Cytoplasm.

Protein Families

CCR4/nocturin family

Tissue Specificity

Widely expressed. Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum.

Database Links

HGNC: 17768

OMIM: 605764

KEGG: hsa:51567

STRING: 9606.ENSP00000367440

UniGene: Hs.403010

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Code	CSB-PA386522
Size	US$166
	Order now
Image	The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA386522(TDP2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA386522(TDP2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, 293T cells, mouse liver tissue, Primary antibody: CSB-PA386522(TDP2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 6 minutes
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TDP2 Antibody

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Target Background