ECR1 Antibody

Code CSB-PA025414XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ECR1 Polyclonal antibody
Uniprot No.
Target Names
ECR1
Alternative Names
ECR1 antibody; At5g19180 antibody; T24G5_80 antibody; NEDD8-activating enzyme E1 catalytic subunit antibody; EC 6.2.1.- antibody; RUB-activating enzyme antibody; Ubiquitin-activating enzyme E1-like protein antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ECR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1.
Gene References into Functions
  1. AXR1 and AXL1 have similar abilities to function with E1 C-TERMINAL-RELATED1 (ECR1) in catalyzing RUB1 activation and RUB1-ECR1 thioester formation. PMID: 21311953
  2. The ecr1-1 mutant is resistant to the auxin-like compound indole-3-propionic acid, produces fewer lateral roots than wild type, displays reduced adult height, and stabilizes a reporter fusion protein that is degraded in response to auxin. PMID: 17449645
Subcellular Location
Nucleus.
Protein Families
Ubiquitin-activating E1 family, UBA3 subfamily
Tissue Specificity
Expressed in shoot, root and floral meristems, in vascular tissues of cotyledons and mature leaves, and in the stele of the root.
Database Links

KEGG: ath:AT5G19180

STRING: 3702.AT5G19180.1

UniGene: At.21297

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