rfl-1 Antibody

Datasheet

Code	CSB-PA613898XA01CXY
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Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans rfl-1 Polyclonal antibody

Uniprot No.

Q19360

Target Names

rfl-1

Alternative Names

rfl-1 antibody; uba-3 antibody; F11H8.1 antibody; NEDD8-activating enzyme E1 catalytic subunit antibody; EC 6.2.1.- antibody; Ectopic membrane ruffles in embryo protein 1 antibody; Ubiquitin-activating enzyme 3 homolog antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans rfl-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Catalytic subunit of the dimeric rfl-1 (uba-3)-ula-1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uba-3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12. Required for cytokinesis and mitotic spindle orientation during early embryogenesis.

Gene References into Functions

Depletion of the strongest specific suppressors rescued the early embryonic cell division defects in rfl-1 mutants; depletion of the two strongest specific enhancers did not affect the early embryonic cell division defects PMID: 19528325

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Ubiquitin-activating E1 family, UBA3 subfamily

Tissue Specificity

Expressed in intestine, vulva epithelium and head and tail neurons.

Database Links

KEGG: cel:CELE_F11H8.1

STRING: 6239.F11H8.1.1

UniGene: Cel.10144

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rfl-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background