Recombinant NEDD8-activating enzyme E1 catalytic subunit (uba-3)

Code CSB-YP613898CXY
MSDS
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Source Yeast
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Code CSB-EP613898CXY
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Source E.coli
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Code CSB-EP613898CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613898CXY
MSDS
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Source Baculovirus
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Code CSB-MP613898CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rfl-1
Uniprot No.
Alternative Names
rfl-1; uba-3; F11H8.1; NEDD8-activating enzyme E1 catalytic subunit; EC 6.2.1.-; Ectopic membrane ruffles in embryo protein 1; Ubiquitin-activating enzyme 3 homolog
Species
Caenorhabditis elegans
Expression Region
1-430
Target Protein Sequence
MVSVDPLATE RWRSIRRLTD RDSAYKVPWF VPGPENFEAL QNTKILVIGA GGLGCELLKN LALSGFRTIE VIDMDTIDVS NLNRQFLFRE SDVGKSKAEV AAAFVQQRVV GCQVTAHNCR IEDKGQEFYR KFSIIICGLD SIPARRWING MLCDLVLEMA DGKPDENTII PMIDGGTEGF KGNARVIYPK FTACIDCTLD LYPPQVNFPL CTIAHTPRLP EHCIEYIKVV VWPEEKPFEG VSLDADDPIH VEWVLERASL RAEKYNIRGV DRRLTSGVLK RIIPAVASTN AVIAASCALE ALKLATNIAK PIDNYLNFTQ IHGAYTSVVS MMKDDNCLTC SGGRLPFEVS PSSTLESLII RLSERFHLKH PTLATSTRKL YCISSFMPQF EQESKENLHT SMKDLVSDGE EILVSDEALS RALTLRIQLI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic subunit of the dimeric rfl-1 (uba-3)-ula-1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uba-3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12. Required for cytokinesis and mitotic spindle orientation during early embryogenesis.
Gene References into Functions
  1. Depletion of the strongest specific suppressors rescued the early embryonic cell division defects in rfl-1 mutants; depletion of the two strongest specific enhancers did not affect the early embryonic cell division defects PMID: 19528325
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Ubiquitin-activating E1 family, UBA3 subfamily
Tissue Specificity
Expressed in intestine, vulva epithelium and head and tail neurons.
Database Links

KEGG: cel:CELE_F11H8.1

STRING: 6239.F11H8.1.1

UniGene: Cel.10144

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