UBR1 Antibody

Code CSB-PA025516XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBR1 Polyclonal antibody
Uniprot No.
Target Names
UBR1
Alternative Names
UBR1 antibody; PTR1 antibody; YGR184C antibody; G7168 antibody; E3 ubiquitin-protein ligase UBR1 antibody; EC 2.3.2.27 antibody; N-end-recognizing protein antibody; N-recognin-1 antibody; RING-type E3 ubiquitin transferase UBR1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) UBR1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
Gene References into Functions
  1. Ubr1 Is Present at the endoplasmic reticulum membrane of wild-type cells and becomes a ligase for ER-associated protein degradation of Ste6* under heat and ethanol stress. PMID: 23988329
  2. Study shows that the RING-type Ubr1 E3 and the HECT-type Ufd4 E3 interact, both physically and functionally. PMID: 21076411
  3. The UBR box, alone & in complex with N-degron peptides, has a previously unknown protein fold stabilized by a novel binuclear zinc center. N-terminal Arg, Lys or His N-degron side chains are coordinated in a multispecific binding pocket. PMID: 20835240
  4. Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway PMID: 18566452
  5. Yck1/Yck2-mediated phosphorylation of Ubr1 on Ser(300) plays a major role in the control of peptide import by the N-end rule pathway, the subsequent (primed) phosphorylations, including the one on Tyr(277), have at most minor effects on Ubr1 PMID: 19033468
  6. Ubr1 is responsible for targeting misfolded cytosoplasmic protein to proteasomal degradation. PMID: 19041308

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Protein Families
UBR1 family
Database Links

KEGG: sce:YGR184C

STRING: 4932.YGR184C

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