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Recombinant Saccharomyces cerevisiae E3 ubiquitin-protein ligase UBR1 (UBR1), partial

Recombinant Saccharomyces cerevisiae E3 ubiquitin-protein ligase UBR1 (UBR1), partial

Code	CSB-YP025516SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP025516SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP025516SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP025516SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP025516SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

UBR1

Uniprot No.

P19812

Alternative Names

UBR1; PTR1; YGR184C; G7168; E3 ubiquitin-protein ligase UBR1; EC 2.3.2.27; N-end-recognizing protein; N-recognin-1; RING-type E3 ubiquitin transferase UBR1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.

Gene References into Functions

Ubr1 Is Present at the endoplasmic reticulum membrane of wild-type cells and becomes a ligase for ER-associated protein degradation of Ste6* under heat and ethanol stress. PMID: 23988329
Study shows that the RING-type Ubr1 E3 and the HECT-type Ufd4 E3 interact, both physically and functionally. PMID: 21076411
The UBR box, alone & in complex with N-degron peptides, has a previously unknown protein fold stabilized by a novel binuclear zinc center. N-terminal Arg, Lys or His N-degron side chains are coordinated in a multispecific binding pocket. PMID: 20835240
Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway PMID: 18566452
Yck1/Yck2-mediated phosphorylation of Ubr1 on Ser(300) plays a major role in the control of peptide import by the N-end rule pathway, the subsequent (primed) phosphorylations, including the one on Tyr(277), have at most minor effects on Ubr1 PMID: 19033468
Ubr1 is responsible for targeting misfolded cytosoplasmic protein to proteasomal degradation. PMID: 19041308