ufm-1 Antibody

Code CSB-PA025560XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans ufm-1 Polyclonal antibody
Uniprot No.
Target Names
ufm-1
Alternative Names
ufm-1 antibody; tag-277 antibody; ZK652.3 antibody; Ubiquitin-fold modifier 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans ufm-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation requires the ufm-1-activating E1 enzyme uba-5, the ufm-1-conjugating E2 enzyme ufc-1, and probably the ufm-1-ligase E3 enzyme ufl-1.
Gene References into Functions
  1. Data report the crystal structure of Ufm1 complexed with a catalytically inactive mutant of UfSP. The structure shows that in addition to the three terminal residues of cUfm1, Pro88-Arg89-Asp90 corresponding to P6-P4 positions from the cleavage site, which are conserved and unique to Ufm1, makes extensive interactions with UfSP. PMID: 29251776
  2. Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy PMID: 27545681
Protein Families
UFM1 family
Tissue Specificity
Expressed in the intestine and head neurons.
Database Links

KEGG: cel:CELE_ZK652.3

STRING: 6239.ZK652.3.2

UniGene: Cel.19419

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