Recombinant Caenorhabditis elegans Ubiquitin-fold modifier 1 (tag-277)

Code CSB-YP025560CXY
MSDS
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Source Yeast
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Code CSB-EP025560CXY
MSDS
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Source E.coli
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Code CSB-EP025560CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025560CXY
MSDS
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Source Baculovirus
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Code CSB-MP025560CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ufm-1
Uniprot No.
Alternative Names
ufm-1; tag-277; ZK652.3; Ubiquitin-fold modifier 1
Species
Caenorhabditis elegans
Expression Region
1-93
Target Protein Sequence
MSGGTAATTA GSKVTFKITL TSDPKLPFKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGVGVNPA QPAGNIFLKH GSELRLIPRD RVG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation requires the ufm-1-activating E1 enzyme uba-5, the ufm-1-conjugating E2 enzyme ufc-1, and probably the ufm-1-ligase E3 enzyme ufl-1.
Gene References into Functions
  1. Data report the crystal structure of Ufm1 complexed with a catalytically inactive mutant of UfSP. The structure shows that in addition to the three terminal residues of cUfm1, Pro88-Arg89-Asp90 corresponding to P6-P4 positions from the cleavage site, which are conserved and unique to Ufm1, makes extensive interactions with UfSP. PMID: 29251776
  2. Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy PMID: 27545681
Protein Families
UFM1 family
Tissue Specificity
Expressed in the intestine and head neurons.
Database Links

KEGG: cel:CELE_ZK652.3

STRING: 6239.ZK652.3.2

UniGene: Cel.19419

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