umuC Antibody

Code CSB-PA366108XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) umuC Polyclonal antibody
Uniprot No.
Target Names
umuC
Alternative Names
umuC antibody; b1184 antibody; JW1173 antibody; Protein UmuC antibody; DNA polymerase V antibody; Pol V antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) umuC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair. Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions.
Gene References into Functions
  1. This work reveals a new level of regulation in the activation of DNA polymerase V. pol V is subject to a novel form of spatial regulation. PMID: 26317348
  2. The UmuC peptide follows the overall disposition of previously characterised structures with respect to the highly conserved glutamine residue. PMID: 23822808
  3. Y11A UmuC mutant has increased UV-sensitivity and reduced UV-mutability due to excessive incorporation of rNTPs during translesion DNA synthesis. PMID: 22784977
  4. Variants of UmuC with amino acid substitutions at, or adjacent to, its steric gate were studied to gain insights into sugar selectivity and overall fidelity of E. coli pol V. PMID: 22422840
  5. In the absence of repair or when the repair capacity of the cell has been exceeded, translesion synthesis by polymerase V (Pol V) allows DNA synthesis to resume and is required to protect the arrested replication fork from degradation PMID: 16199565
  6. Lyase activity intrinsic to polymerase IV PMID: 16202661

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Protein Families
DNA polymerase type-Y family
Database Links
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