Recombinant Escherichia coli Protein umuC (umuC)

Code CSB-YP366108ENV
MSDS
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Source Yeast
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Code CSB-EP366108ENV
MSDS
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Source E.coli
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Code CSB-EP366108ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP366108ENV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP366108ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
umuC
Uniprot No.
Alternative Names
umuC; b1184; JW1173; Protein UmuC; DNA polymerase V; Pol V
Species
Escherichia coli (strain K12)
Expression Region
1-422
Target Protein Sequence
MFALCDVNAF YASCETVFRP DLWGKPVVVL SNNDGCVIAR NAEAKALGVK MGDPWFKQKD LFRRCGVVCF SSNYELYADM SNRVMSTLEE LSPRVEIYSI DEAFCDLTGV RNCRDLTDFG REIRATVLQR THLTVGVGIA QTKTLAKLAN HAAKKWQRQT GGVVDLSNLE RQRKLMSALP VDDVWGIGRR ISKKLDAMGI KTVLDLADTD IRFIRKHFNV VLERTVRELR GEPCLQLEEF APTKQEIICS RSFGERITDY PSMRQAICSY AARAAEKLRS EHQYCRFIST FIKTSPFALN EPYYGNSASV KLLTPTQDSR DIINAATRSL DAIWQAGHRY QKAGVMLGDF FSQGVAQLNL FDDNAPRPGS EQLMTVMDTL NAKEGRGTLY FAGQGIQQQW QMKRAMLSPR YTTRSSDLLR VK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair. Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions.
Gene References into Functions
  1. This work reveals a new level of regulation in the activation of DNA polymerase V. pol V is subject to a novel form of spatial regulation. PMID: 26317348
  2. The UmuC peptide follows the overall disposition of previously characterised structures with respect to the highly conserved glutamine residue. PMID: 23822808
  3. Y11A UmuC mutant has increased UV-sensitivity and reduced UV-mutability due to excessive incorporation of rNTPs during translesion DNA synthesis. PMID: 22784977
  4. Variants of UmuC with amino acid substitutions at, or adjacent to, its steric gate were studied to gain insights into sugar selectivity and overall fidelity of E. coli pol V. PMID: 22422840
  5. In the absence of repair or when the repair capacity of the cell has been exceeded, translesion synthesis by polymerase V (Pol V) allows DNA synthesis to resume and is required to protect the arrested replication fork from degradation PMID: 16199565
  6. Lyase activity intrinsic to polymerase IV PMID: 16202661

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Protein Families
DNA polymerase type-Y family
Database Links
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