Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans unc-84 Polyclonal antibody

Uniprot No.

Q20745

Target Names

unc-84

Alternative Names

unc-84 antibody; F54B11.3Nuclear migration and anchoring protein unc-84 antibody; Uncoordinated protein 84 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans unc-84 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in nuclear migration and anchoring in hypodermal precursor cells. Most likely recruits anc-1 to the nuclear envelope where anc-1 functions to tether the nucleus to the actin cytoskeleton. Component of the unc-83-unc-84 LINC (LInker of Nucleoskeleton and Cytoskeleton) complex where it recruits and interacts with unc-83 to form a bridge connecting the nuclear envelope to the cytoskeleton which allows for nuclear transport along microtubules. Its role in nuclear migration may be in association with lamin, lmn-1. Regulates nuclear migrations in one-cell embryos, controlling the posterior migration of the male pronucleus following fertilization. Not required for centrosome attachment to the nucleus. Plays a role in the maintenance of the nuclear envelope architecture in body wall muscle cells. May be involved in DNA damage repair through an association with zyg-12. Potentially has roles in homologous recombination, double strand break repair and meiotic recombination. Specifically, may in part inhibit non-homologous end joining repair, most likely through recruiting fan-1 to the nucleoplasm, to facilitate the repair of DNA cross-links.

Gene References into Functions

SUN-KASH bridges are only required to maintain nuclear envelope spacing in cells subjected to increased mechanical forces. PMID: 25023515
targeting UNC-84 requires an initial step that actively transports UNC-84 from the peripheral endoplasmic reticulum to the nuclear envelope PMID: 21411627
UNC-84 and UNC-83, the KASH and SUN domain proteins, bridge the nuclear envelope, connecting the nuclear lamina to cytoskeletal components. PMID: 16481402

Subcellular Location

Nucleus inner membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton.

Tissue Specificity

Expressed in all somatic cells. Not expressed in germ cells in the mitotic and transition zones of the gonad. One study shows expression at the beginning of the late pachytene stage in the proximal gonad, but there is no expression in the male germline, s

Database Links

KEGG: cel:CELE_F54B11.3

STRING: 6239.F54B11.3a

UniGene: Cel.17328

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unc-84 Antibody

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