Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans unc-89 Polyclonal antibody

Uniprot No.

O01761

Target Names

unc-89

Alternative Names

unc-89 antibody; C09D1.1 antibody; Muscle M-line assembly protein unc-89 antibody; Obscurin antibody; Uncoordinated protein 89 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans unc-89 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Structural component of the muscle M line which is involved in assembly and organization of sarcomere myofilaments. The large isoform a, isoform b, isoform d and isoform f play an essential role in maintaining the organization of sarcomeres but not myofilament alignment during body wall muscle development whereas the small isoform c and isoform d appear to have a minor role. Isoform b and isoform f are required for the organization of unc-15/paramyosin into sarcomere thick filaments in body wall muscles. By binding mel-26, a substrate adapter of the cul-3 E3 ubiquitin-protein ligase complex, regulates the organization of myosin thick filaments, likely by preventing the degradation of microtubule severing protein mei-1. Acts as guanine nucleotide exchange factor (GEF) for Rho GTPase rho-1 but not ced-10, mig-2 and cdc-42. The large isoforms regulate Ca(2+) signaling during muscle contraction by ensuring the correct localization of sarco-endoplamic reticulum Ca(2+) ATPase sca-1 and ryanodine receptor unc-68. By controlling the contraction and/or organization of pharyngeal muscles, plays a role in the formation of pharyngeal gland cell extension.

Gene References into Functions

study demonstrates that the level of MEI-1 protein is reduced in an unc-89 mutant, indicating that the normal role of UNC-89 is to inhibit the CUL-3/MEL-26 complex toward MEI-1 in striated muscle PMID: 22621901
data implicates UNC-89 in maintaining muscle cell architecture and that this precise organization is essential for optimal calcium mobilization and efficient and effective muscle contraction. PMID: 22768340
Results show that the coding sequence for unc-89 is larger than originally thought, and that the gene encodes at least four major isoforms. PMID: 15313609
Yeast two-hybrid screening using a portion of UNC-89 including PK2, yielded SCPL-1 (small CTD phosphatase-like-1), which contains a C terminal domain (CTD) phosphatase type domain. PMID: 18337465
Results support a model in which the DH-PH region of UNC-89 activates RHO-1 GTPase for organization of myosin filaments in C. elegans muscle cells. PMID: 18801371
SCPL-1 interacts with LIM-9 (FHL), a protein that we first discovered as an interactor of UNC-97 (PINCH) and UNC-96, components of an M-line costamere in nematode muscle. PMID: 19244614

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Subcellular Location

Cytoplasm, myofibril, sarcomere, M line.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family

Tissue Specificity

Expressed in body-wall, pharyngeal muscles and a few muscle cells of the tail (at protein level). Expressed in gonadal myoepithelial sheath cells (at protein level). Isoform c: Expressed in body wall and vulval muscles but not in pharyngeal muscles. Isofo

Database Links

KEGG: cel:CELE_C09D1.1

STRING: 6239.C09D1.1b

UniGene: Cel.7125

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unc-89 Antibody

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