Recombinant Muscle M-line assembly protein unc-89 (unc-89), partial

Code CSB-YP522322CXY
MSDS
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Source Yeast
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Code CSB-EP522322CXY
MSDS
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Source E.coli
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Code CSB-EP522322CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522322CXY
MSDS
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Source Baculovirus
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Code CSB-MP522322CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
unc-89
Uniprot No.
Alternative Names
unc-89; C09D1.1; Muscle M-line assembly protein unc-89; Obscurin; Uncoordinated protein 89
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Structural component of the muscle M line which is involved in assembly and organization of sarcomere myofilaments. The large isoform a, isoform b, isoform d and isoform f play an essential role in maintaining the organization of sarcomeres but not myofilament alignment during body wall muscle development whereas the small isoform c and isoform d appear to have a minor role. Isoform b and isoform f are required for the organization of unc-15/paramyosin into sarcomere thick filaments in body wall muscles. By binding mel-26, a substrate adapter of the cul-3 E3 ubiquitin-protein ligase complex, regulates the organization of myosin thick filaments, likely by preventing the degradation of microtubule severing protein mei-1. Acts as guanine nucleotide exchange factor (GEF) for Rho GTPase rho-1 but not ced-10, mig-2 and cdc-42. The large isoforms regulate Ca(2+) signaling during muscle contraction by ensuring the correct localization of sarco-endoplamic reticulum Ca(2+) ATPase sca-1 and ryanodine receptor unc-68. By controlling the contraction and/or organization of pharyngeal muscles, plays a role in the formation of pharyngeal gland cell extension.
Gene References into Functions
  1. study demonstrates that the level of MEI-1 protein is reduced in an unc-89 mutant, indicating that the normal role of UNC-89 is to inhibit the CUL-3/MEL-26 complex toward MEI-1 in striated muscle PMID: 22621901
  2. data implicates UNC-89 in maintaining muscle cell architecture and that this precise organization is essential for optimal calcium mobilization and efficient and effective muscle contraction. PMID: 22768340
  3. Results show that the coding sequence for unc-89 is larger than originally thought, and that the gene encodes at least four major isoforms. PMID: 15313609
  4. Yeast two-hybrid screening using a portion of UNC-89 including PK2, yielded SCPL-1 (small CTD phosphatase-like-1), which contains a C terminal domain (CTD) phosphatase type domain. PMID: 18337465
  5. Results support a model in which the DH-PH region of UNC-89 activates RHO-1 GTPase for organization of myosin filaments in C. elegans muscle cells. PMID: 18801371
  6. SCPL-1 interacts with LIM-9 (FHL), a protein that we first discovered as an interactor of UNC-97 (PINCH) and UNC-96, components of an M-line costamere in nematode muscle. PMID: 19244614

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Subcellular Location
Cytoplasm, myofibril, sarcomere, M line.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family
Tissue Specificity
Expressed in body-wall, pharyngeal muscles and a few muscle cells of the tail (at protein level). Expressed in gonadal myoepithelial sheath cells (at protein level). Isoform c: Expressed in body wall and vulval muscles but not in pharyngeal muscles. Isofo
Database Links

KEGG: cel:CELE_C09D1.1

STRING: 6239.C09D1.1b

UniGene: Cel.7125

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