Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) vangl2 Polyclonal antibody

Uniprot No.

Q8UVJ6

Target Names

vangl2

Alternative Names

vangl2; stbm; tri; Vang-like protein 2; Protein strabismus; Protein trilobite; Van Gogh-like protein 2

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) vangl2 protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays a role in non-canonical Wnt/planar cell polarity (PCP) signaling to regulate convergent extension cell movements during gastrulation. Acts together with scrib and prickle1 and localizes prickle1 and dvl2/dsh to the plasma membrane. Has an overlapping role with kny during both convergent extension and eye development. In the eye, involved in establishing proper alignment of the anterior neural plate and midline cells expressing shha and shhb/twhh. Has indirect effects on a number of other developmental processes including notochord shape formation, neural progenitor cell morphogenesis, segregation of somites and adaxial cell development. Together with prickle1, required for the posterior (caudal) movement of branchiomotor neurons in the hindbrain independently of, and a few hours after, convergent extension. May be required for cell surface localization of fzd3 and fzd6 in the inner ear.

Gene References into Functions

Results demonstrate that anterior guidance decisions by commissural primary ascending (CoPA) axons are dependent on the function of planar cell polarity genes Fzd3a, Vangl2 and Scribble both prior to and after midline crossing; experiments establish CoPA axons as a model system to investigate the mechanism of planar cell polarity signaling in commissural axon guidance. PMID: 27955617
dynamic Vangl2 intracellular distribution is coordinated with and necessary for the changes in convergence and extension cell behaviors during gastrulation PMID: 26062934
we found that the critical period for Vangl2 function coincides with the initial stages of FBM neuron migration out of rhombomere 4. PMID: 24333599
Vangl2 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function PMID: 23988578
VANGL2 regulates the endocytosis and cell-surface availability of MMP14 in a manner that is dependent on focal adhesion kinase. PMID: 22357946
Vangl2(R259L) behaved like the wild-type allele in overexpression and morpholino knockdown/rescue assays in zebrafish embryos. PMID: 21404367
data suggest that bbs8 and vangl2 act synergistically on cell polarization to establish and maintain the appropriate length and number of cilia in the Kupffer's vesicle and thereby facilitate correct LR asymmetry PMID: 20643117
Vangl2 is required for asymmetric localization and posterior tilting of primary motile cilia at the apical membrane of epithelial cells. PMID: 20305649
show that anterior hindbrain (r2/r3) motor neuron migration appears to be independent of vangl2 and pk1b functions, and suggest that multiple mechanisms regulate motor neuron migration along the anterior-posterior axis of the zebrafish hindbrain PMID: 19937772
The vangl2 gene product mediates cellular interactions that confer directionality on distinct movements during vertebrate embryogenesis. PMID: 12105418

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Vang family

Tissue Specificity

Ubiquitously expressed at the 4-cell stage. In early somitogenesis, becomes more abundant in anterior neural tissue where expression is seen in the neural tube but not in the notochord.

Database Links

KEGG: dre:245949

STRING: 7955.ENSDARP00000031546

UniGene: Dr.91583

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vangl2 Antibody

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