vapC Antibody

Rare Species
Code CSB-PA520879XA01SZB
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes: vapC Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 44 kDa
    Observed band size: 44 kDa
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Product Details

Full Product Name
Rabbit anti-Shigella flexneri vapC Polyclonal antibody
Uniprot No.
Target Names
vapC
Alternative Names
tRNA(fMet)-specific endonuclease VapC (EC 3.1.-.-) (RNase VapC) (Toxin VapC) vapC mvpA stborf2 CP0245
Raised in
Rabbit
Species Reactivity
Shigella flexneri
Immunogen
Recombinant Shigella flexneri vapC protein (1-132aa)
Immunogen Species
Shigella flexneri
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Toxic component of a type II toxin-antitoxin (TA) system. A tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet); these effects are neutralized by expression of cognate antitoxin VapB. The VapB/VapC complex probably regulates transcription of its own promoter.; Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.
Protein Families
PINc/VapC protein family
Database Links

KEGG: sfl:CP0245

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