VPS29 Antibody

Code CSB-PA874518XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VPS29 Polyclonal antibody
Uniprot No.
Target Names
VPS29
Alternative Names
VPS29 antibody; MAG1 antibody; At3g47810 antibody; T23J7.140 antibody; Vacuolar protein sorting-associated protein 29 antibody; Protein MAIGO 1 antibody; Vesicle protein sorting 29 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VPS29 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the PIN1 recycling to the plasma membrane. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane.
Gene References into Functions
  1. VPS29 plays a crucial role in recycling VSRs from the PVC to the trans-Golgi network during the trafficking of soluble proteins to the lytic vacuole . PMID: 23263768
  2. A combination of immunoelectron and fluorescence microscopy show that VPS29p localize to the trans-Golgi network (TGN), which is considered to represent the early endosome of plants. PMID: 19796370
  3. Findings suggest that MAG1/VPS29 protein is involved in recycling a plant receptor for the efficient sorting of seed storage proteins PMID: 16926167
  4. VPS29 is required for endosome homeostasis, PIN protein cycling, and dynamic PIN1 repolarization during plant organ development. PMID: 17889650
Subcellular Location
Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
VPS29 family
Database Links

KEGG: ath:AT3G47810

STRING: 3702.AT3G47810.1

UniGene: At.3249

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