Recombinant Arabidopsis thaliana Vacuolar protein sorting-associated protein 29 (VPS29)

Code CSB-YP874518DOA
MSDS
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Source Yeast
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Code CSB-EP874518DOA
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Source E.coli
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Code CSB-EP874518DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874518DOA
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Source Baculovirus
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Code CSB-MP874518DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VPS29
Uniprot No.
Alternative Names
VPS29; MAG1; At3g47810; T23J7.140; Vacuolar protein sorting-associated protein 29; Protein MAIGO 1; Vesicle protein sorting 29
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-190
Target Protein Sequence
MVLVLALGDL HVPHRAADLP PKFKSMLVPG KIQHIICTGN LCIKEIHDYL KTICPDLHIV RGEFDEDARY PENKTLTIGQ FKLGLCHGHQ VIPWGDLDSL AMLQRQLGVD ILVTGHTHQF TAYKHEGGVV INPGSATGAY SSINQDVNPS FVLMDIDGFR AVVYVYELID GEVKVDKIEF KKPPTTSSGP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the PIN1 recycling to the plasma membrane. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane.
Gene References into Functions
  1. VPS29 plays a crucial role in recycling VSRs from the PVC to the trans-Golgi network during the trafficking of soluble proteins to the lytic vacuole . PMID: 23263768
  2. A combination of immunoelectron and fluorescence microscopy show that VPS29p localize to the trans-Golgi network (TGN), which is considered to represent the early endosome of plants. PMID: 19796370
  3. Findings suggest that MAG1/VPS29 protein is involved in recycling a plant receptor for the efficient sorting of seed storage proteins PMID: 16926167
  4. VPS29 is required for endosome homeostasis, PIN protein cycling, and dynamic PIN1 repolarization during plant organ development. PMID: 17889650
Subcellular Location
Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
VPS29 family
Database Links

KEGG: ath:AT3G47810

STRING: 3702.AT3G47810.1

UniGene: At.3249

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