VPS34 Antibody

^{Rare Species}

Datasheet

Code	CSB-PA334903XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes:VPS34 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 30 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VPS34 Polyclonal antibody

Uniprot No.

P22543

Target Names

VPS34

Alternative Names

Phosphatidylinositol 3-kinase VPS34 (PI3-kinase VPS34) (PI3K VPS34) (PtdIns-3-kinase VPS34) (EC 2.7.1.137) (Carboxypeptidase Y-deficient protein 15) (Vacuolar protein sorting-associated protein 34) (Vacuolar protein-targeting protein 29) VPS34 END12 PEP15 VPL7 VPT29 YLR240W L9672.10

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VPS34 protein (593-859aa)

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Its activation by VPS15 may lead to the phosphorylation of phosphatidylinositol in the sorting compartment membrane. Finally, it might also be involved in ethanol tolerance and cell wall integrity.

Gene References into Functions

Data indicate that class Ia Phosphatidylinositol 3-Kinase p85alpha germ line mutations associated with SHORT syndrome did not increase PI3K activity in the yeast model. PMID: 28143957
mutants lacking Vps34 display multiple phenotypes indicating impaired transcription elongation. PMID: 23335340
VPS34 plays a critical role in the decline of extracellular FBPase in response to glucose. PMID: 22833678
Kes1-mediated regulation of the autophagy/cytoplasm to the vacuole trafficking pathway is prevented by increasing expression of the PI3K Vps34 PMID: 20729555
Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate. PMID: 16839886
Study shows that the lipid-kinase activity of Vps34 is required for autophagy, implying an essential role of its product PtdIns(3)P. PMID: 18533003

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Subcellular Location

Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.

Protein Families

PI3/PI4-kinase family

Database Links

KEGG: sce:YLR240W

STRING: 4932.YLR240W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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VPS34 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background