Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VSR1 Polyclonal antibody

Uniprot No.

P93026

Target Names

VSR1

Alternative Names

VSR1 antibody; BP80B antibody; ELP antibody; ELP1 antibody; At3g52850 antibody; F8J2.20 antibody; Vacuolar-sorting receptor 1 antibody; AtVSR1 antibody; BP80-like protein b antibody; AtBP80b antibody; Epidermal growth factor receptor-like protein 1 antibody; AtELP antibody; AtELP1 antibody; Spot 3 protein antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana VSR1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles.

Gene References into Functions

VSR1 interacts with adaptor complex AP-4 at the trans-Golgi network and participates in the vacuolar protein sorting. PMID: 26546666
The crystal structures of the protease-associated domain of VSR isoform 1 from Arabidopsis thaliana (VSR1PA) alone and complexed with a cognate peptide containing the barley (Hordeum vulgare) aleurain, are reported. PMID: 25271241
N-glycosylation of AtVSR1 plays a critical role in its function as a Vacuolar sorting receptor (VSR) in plants. PMID: 25293377
required for osmotic stress-induced abscisic acid biosynthesis PMID: 25416474
In mag1-1 mutants, VSR1 failed to recycle from the PVC to the TGN; rather, a significant proportion was transported to the LV; VSR1 overexpression rescued this defect PMID: 23263768
findings show that that VSR1, VSR3 and VSR4 participate in vacuolar sorting of VAC2 in vegetative tissues, and 12S globulins and 2S albumins in seeds PMID: 20807215
These data suggest that homomeric interaction of AtVSR1 is critical for its function as a vacuolar sorting receptor. PMID: 20625000

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Subcellular Location

Membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Single-pass type I membrane protein. Prevacuolar compartment membrane; Single-pass type I membrane protein. Note=Associated to the Golgi apparatus, mostly on the trans-side, to clathrin-coated vesicles (CCVs) and to prevacuolar compartments (PVCs). May also be associated with the plasma membrane.

Protein Families

VSR (BP-80) family

Tissue Specificity

Mostly expressed in roots and dividing cells (at protein level). Also expressed in seeds, seedlings, leaves, stems, flowers, siliques and pollen.

Database Links

KEGG: ath:AT3G52850

STRING: 3702.AT3G52850.1

UniGene: At.24701

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VSR1 Antibody

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