Recombinant Arabidopsis thaliana Vacuolar-sorting receptor 1 (VSR1), partial

Code CSB-YP311285DOA
MSDS
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Source Yeast
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Code CSB-EP311285DOA
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Source E.coli
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Code CSB-EP311285DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP311285DOA
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Source Baculovirus
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Code CSB-MP311285DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VSR1
Uniprot No.
Alternative Names
VSR1; BP80B; ELP; ELP1; At3g52850; F8J2.20; Vacuolar-sorting receptor 1; AtVSR1; BP80-like protein b; AtBP80b; Epidermal growth factor receptor-like protein 1; AtELP; AtELP1; Spot 3 protein
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles.
Gene References into Functions
  1. VSR1 interacts with adaptor complex AP-4 at the trans-Golgi network and participates in the vacuolar protein sorting. PMID: 26546666
  2. The crystal structures of the protease-associated domain of VSR isoform 1 from Arabidopsis thaliana (VSR1PA) alone and complexed with a cognate peptide containing the barley (Hordeum vulgare) aleurain, are reported. PMID: 25271241
  3. N-glycosylation of AtVSR1 plays a critical role in its function as a Vacuolar sorting receptor (VSR) in plants. PMID: 25293377
  4. required for osmotic stress-induced abscisic acid biosynthesis PMID: 25416474
  5. In mag1-1 mutants, VSR1 failed to recycle from the PVC to the TGN; rather, a significant proportion was transported to the LV; VSR1 overexpression rescued this defect PMID: 23263768
  6. findings show that that VSR1, VSR3 and VSR4 participate in vacuolar sorting of VAC2 in vegetative tissues, and 12S globulins and 2S albumins in seeds PMID: 20807215
  7. These data suggest that homomeric interaction of AtVSR1 is critical for its function as a vacuolar sorting receptor. PMID: 20625000

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Subcellular Location
Membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Single-pass type I membrane protein. Prevacuolar compartment membrane; Single-pass type I membrane protein. Note=Associated to the Golgi apparatus, mostly on the trans-side, to clathrin-coated vesicles (CCVs) and to prevacuolar compartments (PVCs). May also be associated with the plasma membrane.
Protein Families
VSR (BP-80) family
Tissue Specificity
Mostly expressed in roots and dividing cells (at protein level). Also expressed in seeds, seedlings, leaves, stems, flowers, siliques and pollen.
Database Links

KEGG: ath:AT3G52850

STRING: 3702.AT3G52850.1

UniGene: At.24701

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