VTI11 Antibody

Code CSB-PA890321XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VTI11 Polyclonal antibody
Uniprot No.
Target Names
VTI11
Alternative Names
VTI11 antibody; SGR4 antibody; VTI1A antibody; ZIG antibody; ZIG1 antibody; At5g39510 antibody; MUL8_190 antibody; Vesicle transport v-SNARE 11 antibody; AtVTI11 antibody; Protein SHOOT GRAVITROPISM 4 antibody; Vesicle soluble NSF attachment protein receptor VTI1a antibody; AtVTI1a antibody; Vesicle transport v-SNARE protein VTI1a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VTI11 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May function as a v-SNARE responsible for targeting AtELP-containing vesicles from the trans-Golgi network (TGN) to the prevacuolar compartment (PVC). May be also involved in retrograde traffic to the cis-Golgi. Promotes the formation of vacuolar membrane 'bulbs'. Required for amyloplast sedimentation in the endodermis during shoot gravitropism, which are thus acting as statoliths. Expression in the endodermis is essential for the shoot gravitropic response, whereas expression in other tissues may be responsible for the correct stem and leaf shape.
Gene References into Functions
  1. The vacuoles in itt3/vti11 are independent organelles. Results provided supporting evidence for a critical role of PtdIns(3)P in vacuole fusion in roots and guard cells. [ITT3] PMID: 25482812
  2. Homotypic vacuole fusion requires VTI11 and is regulated by phosphoinositides. PMID: 24569132
  3. The frequency of bulbs is affected in the three sgr mutants sgr2, sgr3 and sgr4/zig. PMID: 21645145
  4. Functional deficiency of the retromer large subunit suppresses the loss-of-function mutant of Qb-SNARE VTI11, zig-1. PMID: 20086190
Subcellular Location
Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein. Prevacuolar compartment membrane; Single-pass type IV membrane protein. Vacuole membrane; Single-pass type IV membrane protein.
Protein Families
VTI1 family
Tissue Specificity
Expressed in roots, stems, flowers and leaves.
Database Links

KEGG: ath:AT5G39510

STRING: 3702.AT5G39510.1

UniGene: At.30339

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