Recombinant Arabidopsis thaliana Vesicle transport v-SNARE 11 (VTI11), partial

Code CSB-YP890321DOA
MSDS
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Source Yeast
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Code CSB-EP890321DOA
MSDS
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Source E.coli
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Code CSB-EP890321DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890321DOA
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Source Baculovirus
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Code CSB-MP890321DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VTI11
Uniprot No.
Alternative Names
VTI11; SGR4; VTI1A; ZIG; ZIG1; At5g39510; MUL8_190; Vesicle transport v-SNARE 11; AtVTI11; Protein SHOOT GRAVITROPISM 4; Vesicle soluble NSF attachment protein receptor VTI1a; AtVTI1a; Vesicle transport v-SNARE protein VTI1a
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May function as a v-SNARE responsible for targeting AtELP-containing vesicles from the trans-Golgi network (TGN) to the prevacuolar compartment (PVC). May be also involved in retrograde traffic to the cis-Golgi. Promotes the formation of vacuolar membrane 'bulbs'. Required for amyloplast sedimentation in the endodermis during shoot gravitropism, which are thus acting as statoliths. Expression in the endodermis is essential for the shoot gravitropic response, whereas expression in other tissues may be responsible for the correct stem and leaf shape.
Gene References into Functions
  1. The vacuoles in itt3/vti11 are independent organelles. Results provided supporting evidence for a critical role of PtdIns(3)P in vacuole fusion in roots and guard cells. [ITT3] PMID: 25482812
  2. Homotypic vacuole fusion requires VTI11 and is regulated by phosphoinositides. PMID: 24569132
  3. The frequency of bulbs is affected in the three sgr mutants sgr2, sgr3 and sgr4/zig. PMID: 21645145
  4. Functional deficiency of the retromer large subunit suppresses the loss-of-function mutant of Qb-SNARE VTI11, zig-1. PMID: 20086190
Subcellular Location
Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein. Prevacuolar compartment membrane; Single-pass type IV membrane protein. Vacuole membrane; Single-pass type IV membrane protein.
Protein Families
VTI1 family
Tissue Specificity
Expressed in roots, stems, flowers and leaves.
Database Links

KEGG: ath:AT5G39510

STRING: 3702.AT5G39510.1

UniGene: At.30339

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