WER Antibody

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Code	CSB-PA879724XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WER Polyclonal antibody

Uniprot No.

Q9SEI0

Target Names

WER

Alternative Names

WER antibody; MYB66 antibody; At5g14750 antibody; T9L3.50 antibody; Transcription factor WER antibody; Myb-related protein 66 antibody; AtMYB66 antibody; Protein WEREWOLF antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana WER protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in roots and hypocotyl. Together with GL3 or BHLH2, promotes the formation of non-hair developing cells (atrichoblasts) et the N position in root epidermis. Regulates stomata spatial distribution in hypocotyls. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes such as GL2 and of CPC.

Gene References into Functions

TRN1 regulates the position-dependent cell fate determination by affecting WER expression in Arabidopsis root epidermis. PMID: 26451798
WER expressed ubiquitously in the root induced GL2 expression only in the root epidermis, whereas co-expression of WER and EGL3 induced GL2 expression in the corresponding tissues. PMID: 26408906
Phosphatidic acid interacts with werewolf (WER), a R2R3 MYB transcription factor involved in root hair formation. PMID: 24368785
AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity. PMID: 25428588
MYB23, WER, and MYB23-WER chimeric protein have similar functions in respect to root hair formation. PMID: 22738953
WER regulates not the transcription but the stability of FT mRNA. PMID: 21653190

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Subcellular Location

Nucleus.

Tissue Specificity

Restricted to roots and hypocotyl. Specifically expressed in root non-hair developing cells (atrichoblasts) at the N position. Also present in lateral root cap cells. In hypocotyls, expressed within files of epidermal cells located outside a single cortic

Database Links

KEGG: ath:AT5G14750

STRING: 3702.AT5G14750.1

UniGene: At.28680

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WER Antibody

Product Details

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Target Background