Recombinant Arabidopsis thaliana Transcription factor WER (WER)

Code CSB-YP879724DOA
MSDS
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Source Yeast
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Code CSB-EP879724DOA
MSDS
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Source E.coli
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Code CSB-EP879724DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879724DOA
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Source Baculovirus
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Code CSB-MP879724DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WER
Uniprot No.
Alternative Names
WER; MYB66; At5g14750; T9L3.50; Transcription factor WER; Myb-related protein 66; AtMYB66; Protein WEREWOLF
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-203
Target Protein Sequence
MRKKVSSSGD EGNNEYKKGL WTVEEDKILM DYVKAHGKGH WNRIAKKTGL KRCGKSCRLR WMNYLSPNVK RGNFTEQEED LIIRLHKLLG NRWSLIAKRV PGRTDNQVKN YWNTHLSKKL GIKDQKTKQS NGDIVYQINL PNPTETSEET KISNIVDNNN ILGDEIQEDH QGSNYLSSLW VHEDEFELST LTNMMDFIDG HCF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in roots and hypocotyl. Together with GL3 or BHLH2, promotes the formation of non-hair developing cells (atrichoblasts) et the N position in root epidermis. Regulates stomata spatial distribution in hypocotyls. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes such as GL2 and of CPC.
Gene References into Functions
  1. TRN1 regulates the position-dependent cell fate determination by affecting WER expression in Arabidopsis root epidermis. PMID: 26451798
  2. WER expressed ubiquitously in the root induced GL2 expression only in the root epidermis, whereas co-expression of WER and EGL3 induced GL2 expression in the corresponding tissues. PMID: 26408906
  3. Phosphatidic acid interacts with werewolf (WER), a R2R3 MYB transcription factor involved in root hair formation. PMID: 24368785
  4. AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity. PMID: 25428588
  5. MYB23, WER, and MYB23-WER chimeric protein have similar functions in respect to root hair formation. PMID: 22738953
  6. WER regulates not the transcription but the stability of FT mRNA. PMID: 21653190

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Subcellular Location
Nucleus.
Tissue Specificity
Restricted to roots and hypocotyl. Specifically expressed in root non-hair developing cells (atrichoblasts) at the N position. Also present in lateral root cap cells. In hypocotyls, expressed within files of epidermal cells located outside a single cortic
Database Links

KEGG: ath:AT5G14750

STRING: 3702.AT5G14750.1

UniGene: At.28680

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