Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WRKY46 Polyclonal antibody

Uniprot No.

Q9SKD9

Target Names

WRKY46

Alternative Names

WRKY46 antibody; At2g46400 antibody; F11C10.9 antibody; Probable WRKY transcription factor 46 antibody; WRKY DNA-binding protein 46 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana WRKY46 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor involved in the regulation of osmotic stress responses and stomatal movement. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positive regulator of EDS1-dependent defense against E.amylovora. Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2. Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression.

Gene References into Functions

WRKY46 plays an important role in the control of root-to-shoot iron translocation under iron deficiency condition via direct regulation of VITL1 transcript levels.WRKY46 binds to the promoter region of VITL1. PMID: 27208259
results demonstrate that WRKY46 contributes to the feedforward inhibition of osmotic/salt stress-dependent LR inhibition via regulation of ABA signaling and auxin homeostasis PMID: 26252246
WRKY46 plays dual roles in regulating plant responses to drought and salt stress and light-dependent stomatal opening in guard cells. PMID: 24773321
WRKY46 functions in Al sensitivity through regulating the expression of ALMT1. Mutation of WRKY46 leads to increased Al resistance. PMID: 24118304
WRKY46, WRKY70, and WRKY53 positively regulate basal resistance to Pseudomonas syringae; and that they play overlapping and synergetic roles in plant basal defense. PMID: 22325892
WRKY28 and WRKY46 are transcriptional activators of ICS1 and PBS3, respectively, and regulate salicylic acid synthesis upon pathogen attack. [WRKY46] PMID: 21595875

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Subcellular Location

Nucleus.

Protein Families

WRKY group III family

Tissue Specificity

Expressed in guard cells, hypocotyls, and in the vascular tissues of cotyledon and root. Mostly expressed in roots, at lower levels in leaves and petioles, and, to a lower extent, in stems, flowers and siliques.

Database Links

KEGG: ath:AT2G46400

STRING: 3702.AT2G46400.1

UniGene: At.26510

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WRKY46 Antibody

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Target Background