Recombinant Arabidopsis thaliana Probable WRKY transcription factor 46 (WRKY46)

Code CSB-YP886713DOA
MSDS
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Source Yeast
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Code CSB-EP886713DOA
MSDS
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Source E.coli
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Code CSB-EP886713DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886713DOA
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Source Baculovirus
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Code CSB-MP886713DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WRKY46
Uniprot No.
Alternative Names
WRKY46; At2g46400; F11C10.9; Probable WRKY transcription factor 46; WRKY DNA-binding protein 46
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-295
Target Protein Sequence
MMMEEKLVIN ELELGKELAN RLMNNLKHTS SVDSNKTLIS DILRIYQNAI FMLSFNQDKN ILKRSLEIDG KDSKNVFKKR KVSEKNTEKV KVFVATEQEN GSIDDGHCWR KYGQKEIHGS KNPRAYYRCT HRFTQDCLAV KQVQKSDTDP SLFEVKYLGN HTCNNITSPK TTTNFSVSLT NTNIFEGNRV HVTEQSEDMK PTKSEEVMIS LEDLENKKNI FRTFSFSNHE IENGVWKSNL FLGNFVEDLS PATSGSAITS EVLSAPAAVE NSETADSYFS SLDNIIDFGQ DWLWS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the regulation of osmotic stress responses and stomatal movement. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positive regulator of EDS1-dependent defense against E.amylovora. Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2. Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression.
Gene References into Functions
  1. WRKY46 plays an important role in the control of root-to-shoot iron translocation under iron deficiency condition via direct regulation of VITL1 transcript levels.WRKY46 binds to the promoter region of VITL1. PMID: 27208259
  2. results demonstrate that WRKY46 contributes to the feedforward inhibition of osmotic/salt stress-dependent LR inhibition via regulation of ABA signaling and auxin homeostasis PMID: 26252246
  3. WRKY46 plays dual roles in regulating plant responses to drought and salt stress and light-dependent stomatal opening in guard cells. PMID: 24773321
  4. WRKY46 functions in Al sensitivity through regulating the expression of ALMT1. Mutation of WRKY46 leads to increased Al resistance. PMID: 24118304
  5. WRKY46, WRKY70, and WRKY53 positively regulate basal resistance to Pseudomonas syringae; and that they play overlapping and synergetic roles in plant basal defense. PMID: 22325892
  6. WRKY28 and WRKY46 are transcriptional activators of ICS1 and PBS3, respectively, and regulate salicylic acid synthesis upon pathogen attack. [WRKY46] PMID: 21595875

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Subcellular Location
Nucleus.
Protein Families
WRKY group III family
Tissue Specificity
Expressed in guard cells, hypocotyls, and in the vascular tissues of cotyledon and root. Mostly expressed in roots, at lower levels in leaves and petioles, and, to a lower extent, in stems, flowers and siliques.
Database Links

KEGG: ath:AT2G46400

STRING: 3702.AT2G46400.1

UniGene: At.26510

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