Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WRKY54 Polyclonal antibody

Uniprot No.

Q93WU8

Target Names

WRKY54

Alternative Names

WRKY54 antibody; At2g40750 antibody; T7D17.7 antibody; Probable WRKY transcription factor 54 antibody; WRKY DNA-binding protein 54 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana WRKY54 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Together with WRKY70, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes. Positive regulator of EDS1-dependent defense against E.amylovora. In collaboration with WRKY70, prevents stomatal closure and, consequently, osmotic stress tolerance. Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. Negative regulator of SA biosynthesis. Prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense against biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000, probably by regulating negatively the jasmonic acid (JA)/ethylene (ET) and positively the salicylic acid (SA) signaling pathways.

Gene References into Functions

Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses PMID: 28576847
data suggest that the elevated SA level in the wrky54wrky70 double mutant results in moderate accumulation of H2O2, in promoting cell wall fortification and consequently enhanced resistance to necrotrophs but is not sufficient to trigger hypersensitive reaction (HR)-like cell death and resistance to biotrophs/hemibiotrophs like Pst DC3000 PMID: 28837631
WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling. PMID: 23815736
WRKY54 and WRKY70 are negative regulators of leaf senescence. PMID: 22268143

Subcellular Location

Nucleus.

Protein Families

WRKY group III family

Tissue Specificity

Expressed in leaves.

Database Links

KEGG: ath:AT2G40750

STRING: 3702.AT2G40750.1

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WRKY54 Antibody

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