Recombinant Arabidopsis thaliana Probable WRKY transcription factor 54 (WRKY54)

Code CSB-YP853161DOA
MSDS
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Source Yeast
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Code CSB-EP853161DOA
MSDS
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Source E.coli
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Code CSB-EP853161DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853161DOA
MSDS
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Source Baculovirus
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Code CSB-MP853161DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WRKY54
Uniprot No.
Alternative Names
WRKY54; At2g40750; T7D17.7; Probable WRKY transcription factor 54; WRKY DNA-binding protein 54
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-346
Target Protein Sequence
MDSNSNNTKS IKRKVVDQLV EGYEFATQLQ LLLSHQHSNQ YHIDETRLVS GSGSVSGGPD PVDELMSKIL GSFHKTISVL DSFDPVAVSV PIAVEGSWNA SCGDDSATPV SCNGGDSGES KKKRLGVGKG KRGCYTRKTR SHTRIVEAKS SEDRYAWRKY GQKEILNTTF PRSYFRCTHK PTQGCKATKQ VQKQDQDSEM FQITYIGYHT CTANDQTHAK TEPFDQEIIM DSEKTLAAST AQNHVNAMVQ EQENNTSSVT AIDAGMVKEE QNNNGDQSKD YYEGSSTGED LSLVWQETMM FDDHQNHYYC GETSTTSHQF GFIDNDDQFS SFFDSYCADY ERTSAM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Together with WRKY70, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes. Positive regulator of EDS1-dependent defense against E.amylovora. In collaboration with WRKY70, prevents stomatal closure and, consequently, osmotic stress tolerance. Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. Negative regulator of SA biosynthesis. Prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense against biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000, probably by regulating negatively the jasmonic acid (JA)/ethylene (ET) and positively the salicylic acid (SA) signaling pathways.
Gene References into Functions
  1. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses PMID: 28576847
  2. data suggest that the elevated SA level in the wrky54wrky70 double mutant results in moderate accumulation of H2O2, in promoting cell wall fortification and consequently enhanced resistance to necrotrophs but is not sufficient to trigger hypersensitive reaction (HR)-like cell death and resistance to biotrophs/hemibiotrophs like Pst DC3000 PMID: 28837631
  3. WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling. PMID: 23815736
  4. WRKY54 and WRKY70 are negative regulators of leaf senescence. PMID: 22268143
Subcellular Location
Nucleus.
Protein Families
WRKY group III family
Tissue Specificity
Expressed in leaves.
Database Links
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