wun Antibody

Code CSB-PA893233XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) wun Polyclonal antibody
Uniprot No.
Target Names
wun
Alternative Names
wun; CG8804; Putative phosphatidate phosphatase; Germ cell guidance factor; Phosphatidic acid phosphatase type 2; Protein wunen
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) wun protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Responsible for guiding the germ cells early in the process of migration from the lumen of the developing gut towards the overlying mesoderm, where the germ cells enter the gonads. May be involved in lipid metabolism.
Gene References into Functions
  1. Wunen mutants have cardioblast joining defects and loose pericardial cells in the developing heart. PMID: 25224224
  2. The germ cell migratory path can be primarily accounted for by Wunen expression. PMID: 24006260
  3. germ cell survival and directionality of migration are controlled by two lipid phosphate phosphatases (LPP), wunen (wun) and wunen-2 (wun2). wun wun2 double mutant analysis reveals that the two genes act redundantly in primordial germ cells. PMID: 20431117
  4. there are marked differences in biochemical activity between fly Wun and mammalian lipid phosphate phosphatase (LPPs), with Wun having a narrower activity range than has been reported for the mammalian LPPs PMID: 12856002
  5. Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2 have roles in lateral migration and germ cell elimination PMID: 16301333

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
PA-phosphatase related phosphoesterase family
Tissue Specificity
Expressed in embryonic gut in a pattern that guides germ cells towards mesoderm (initially in hindgut and then on lower side of gut). During extended germ band stage, expressed in ectoderm as a medial band throughout the trunk.
Database Links

KEGG: dme:Dmel_CG8804

STRING: 7227.FBpp0087635

UniGene: Dm.3348

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