Recombinant Drosophila melanogaster Putative phosphatidate phosphatase(wun)

Code CSB-CF893233DLU
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names wun
Uniprot No. Q9V576
Alternative Names wun; CG8804; Putative phosphatidate phosphatase; Germ cell guidance factor; Phosphatidic acid phosphatase type 2; Protein wunen
Species Drosophila melanogaster (Fruit fly)
Expression Region 1-379
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Responsible for guiding the germ cells early in the process of migration from the lumen of the developing gut towards the overlying mesoderm, where the germ cells enter the gonads. May be involved in lipid metabolism.
Gene References into Functions
  1. Wunen mutants have cardioblast joining defects and loose pericardial cells in the developing heart. PMID: 25224224
  2. The germ cell migratory path can be primarily accounted for by Wunen expression. PMID: 24006260
  3. germ cell survival and directionality of migration are controlled by two lipid phosphate phosphatases (LPP), wunen (wun) and wunen-2 (wun2). wun wun2 double mutant analysis reveals that the two genes act redundantly in primordial germ cells. PMID: 20431117
  4. there are marked differences in biochemical activity between fly Wun and mammalian lipid phosphate phosphatase (LPPs), with Wun having a narrower activity range than has been reported for the mammalian LPPs PMID: 12856002
  5. Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2 have roles in lateral migration and germ cell elimination PMID: 16301333

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Subcellular Location Membrane; Multi-pass membrane protein.
Protein Families PA-phosphatase related phosphoesterase family
Tissue Specificity Expressed in embryonic gut in a pattern that guides germ cells towards mesoderm (initially in hindgut and then on lower side of gut). During extended germ band stage, expressed in ectoderm as a medial band throughout the trunk.
Database Links

KEGG: dme:Dmel_CG8804

STRING: 7227.FBpp0087635

UniGene: Dm.3348

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