yafQ Antibody

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Code	CSB-PA675091XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) yafQ Polyclonal antibody

Uniprot No.

Q47149

Target Names

yafQ

Alternative Names

yafQ antibody; b0225 antibody; JW0215 antibody; mRNA interferase toxin YafQ antibody; EC 3.1.-.- antibody; Endoribonuclease YafQ antibody; Toxin YafQ antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) yafQ protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Toxic component of a type II toxin-antitoxin (TA) system. A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis. Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs. Ribosome-binding confers the sequence specificity and reading frame-dependence. When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with cognate antitoxin DinJ. YafQ and DinJ together bind their own promoter, and repress its expression.; Cell death governed by the MazE-MazF and DinJ-YafQ TA systems seems to play a role in biofilm formation.

Gene References into Functions

The findings demonstrate the plasticity of ribosome-dependent toxin active site residues by the E. coli YafQ toxin and further our understanding of which toxin residues are important for function. PMID: 26261214
YafQ increases persistence by reducing indole, and toxin/antitoxin systems are related to cell signalling. PMID: 25041421
The endoribonuclease activity of YafQ was inhibited in the complex with DinJ antitoxin in vitro. PMID: 22247505
Acts as toxin in active toxin-antitoxin module with DinJ. Structure modelling suggests that it might act as a sequence-specific mRNA endoribonuclease. PMID: 17263853
YafQ endoribonuclease expression resulted in translation, but not transcription or replication defects. PMID: 19210620

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Protein Families

RelE toxin family, YafQ subfamily

Database Links

KEGG: ecj:JW0215

STRING: 316385.ECDH10B_0207

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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yafQ Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background