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Recombinant Escherichia coli mRNA interferase YafQ (yafQ)

Recombinant Escherichia coli mRNA interferase YafQ (yafQ)

Code	CSB-YP675091ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP675091ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP675091ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP675091ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP675091ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

yafQ

Uniprot No.

Q47149

Alternative Names

yafQ; b0225; JW0215; mRNA interferase toxin YafQ; EC 3.1.-.-; Endoribonuclease YafQ; Toxin YafQ

Species

Escherichia coli (strain K12)

Expression Region

1-92

Target Protein Sequence

MIQRDIEYSG QYSKDVKLAQ KRHKDMNKLK YLMTLLINNT LPLPAVYKDH PLQGSWKGYR DAHVEPDWIL IYKLTDKLLR FERTGTHAAL FG

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Toxic component of a type II toxin-antitoxin (TA) system. A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis. Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs. Ribosome-binding confers the sequence specificity and reading frame-dependence. When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with cognate antitoxin DinJ. YafQ and DinJ together bind their own promoter, and repress its expression.; Cell death governed by the MazE-MazF and DinJ-YafQ TA systems seems to play a role in biofilm formation.

Gene References into Functions

The findings demonstrate the plasticity of ribosome-dependent toxin active site residues by the E. coli YafQ toxin and further our understanding of which toxin residues are important for function. PMID: 26261214
YafQ increases persistence by reducing indole, and toxin/antitoxin systems are related to cell signalling. PMID: 25041421
The endoribonuclease activity of YafQ was inhibited in the complex with DinJ antitoxin in vitro. PMID: 22247505
Acts as toxin in active toxin-antitoxin module with DinJ. Structure modelling suggests that it might act as a sequence-specific mRNA endoribonuclease. PMID: 17263853
YafQ endoribonuclease expression resulted in translation, but not transcription or replication defects. PMID: 19210620

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Protein Families

RelE toxin family, YafQ subfamily

Database Links

KEGG: ecj:JW0215

STRING: 316385.ECDH10B_0207

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