Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPT7 Polyclonal antibody

Uniprot No.

P32939

Target Names

YPT7

Alternative Names

YPT7 antibody; VAM4 antibody; YML001W antibody; YM8270.02 antibody; GTP-binding protein YPT7 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPT7 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Ypt/Rab-type GTPases are key regulators of membrane trafficking and intracellular vesicular transport. They act as molecular switches that convert between GTP-bound and GDP-bound states, and regulate virtually all steps of membrane traffic from the formation of the transport vesicle at the donor membrane to its fusion at the target membrane. In the GDP-bound state, Ypt proteins are predominantly cytosolic, solubilized through the interaction with a GDP dissociation inhibitor (GDI). In the GTP-bound state, the proteins are membrane bound and interact with specific effector proteins that select cargo, promote vesicle movement, or verify the correct site of fusion. YPT7 is involved in regulation of vesicular protein transport in exo- and endocytosis. Involved in homotypic vacuole fusion, the last step in the vacuole inheritance process, by interacting in its GTP-bound state on the donor membrane with the large multiprotein tethering complex termed HOPS on the acceptor membrane. Involved in the regulation of transport steps from late endosomes to the vacuole, mediated by interaction in its GTP-bound state on the donor membrane with the large multiprotein tethering complex termed class C-Vps complex on the acceptor membrane. Involved in retromer assembly and cargo export, recognizing the cargo selection complex (CSC). GTP-bound YPT7 recruits CSC to vacuolar membranes via retromer subunit VPS35. Interacts with the HOPS complex subunit VPS39 independent of the HOPS complex at mitochondria-vacuole contact sites (vCLAMPs), providing a physical and metabolic interconnection between the endocytic pathway and mitochondria.

Gene References into Functions

phosphorylation of vacuole protein sorting complex (HOPS) by recombinant Yck3p confers strict requirement for GTP-bound Ypt7p for binding phosphorylated HOPS, for optimal membrane tethering and proteoliposome fusion; guanine nucleotide specificity of the vacuolar fusion Rab Ypt7p is conferred through downstream posttranslational modification of its effector complex PMID: 22787280
yeast retromer trimer antagonizes Ypt7-regulated organelle tethering and fusion of endosomes/vacuoles via recognition of Ypt7 PMID: 22593205
msb3 vacuoles fuse more efficiently than wild-type vacuoles in vitro, suggesting that GAP can also act on Ypt7 PMID: 22593206
Ypt7p plays a role beyond homotypic fusion and protein sorting-Class C Vps complex recruitment in membrane fusion PMID: 22417749
Ypt7 functions at the late endosome to coordinate retromer-mediated recycling with the fusion of late endosomes with vacuoles. PMID: 21062894
We also show that expression of a mutated variant of the YPT7 gene suppresses the sporulation and autophagy defects of ccz1Delta cells to a quantitatively similar level. PMID: 20709422
apparent forward rate constant of 4 soluble N-ethylmaleimide-sensitive factor attachment protein receptors/vacuole protein sorting complex complex assembly is enhanced many thousand-fold by Ypt7p PMID: 17699614
although Ypt7p may contribute to other fusion functions, its central role is to bind the homoytpic fusion and vacuole protein sorting complex to the membrane PMID: 19386605

Hide All

Subcellular Location

Late endosome. Vacuole membrane.

Protein Families

Small GTPase superfamily, Rab family

Database Links

KEGG: sce:YML001W

STRING: 4932.YML001W

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA330211XA01SVG
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

YPT7 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background