Product Details

Purity

>85% (SDS-PAGE)

Target Names

YPT7

Uniprot No.

P32939

Alternative Names

YPT7; VAM4; YML001W; YM8270.02; GTP-binding protein YPT7

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Expression Region

1-208

Target Protein Sequence

MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV TVDGDKVATM QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI KSWRDEFLVH ANVNSPETFP FVILGNKIDA EESKKIVSEK SAQELAKSLG DIPLFLTSAK NAINVDTAFE EIARSALQQN QADTEAFEDD YNDAINIRLD GENNSCSC

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Ypt/Rab-type GTPases are key regulators of membrane trafficking and intracellular vesicular transport. They act as molecular switches that convert between GTP-bound and GDP-bound states, and regulate virtually all steps of membrane traffic from the formation of the transport vesicle at the donor membrane to its fusion at the target membrane. In the GDP-bound state, Ypt proteins are predominantly cytosolic, solubilized through the interaction with a GDP dissociation inhibitor (GDI). In the GTP-bound state, the proteins are membrane bound and interact with specific effector proteins that select cargo, promote vesicle movement, or verify the correct site of fusion. YPT7 is involved in regulation of vesicular protein transport in exo- and endocytosis. Involved in homotypic vacuole fusion, the last step in the vacuole inheritance process, by interacting in its GTP-bound state on the donor membrane with the large multiprotein tethering complex termed HOPS on the acceptor membrane. Involved in the regulation of transport steps from late endosomes to the vacuole, mediated by interaction in its GTP-bound state on the donor membrane with the large multiprotein tethering complex termed class C-Vps complex on the acceptor membrane. Involved in retromer assembly and cargo export, recognizing the cargo selection complex (CSC). GTP-bound YPT7 recruits CSC to vacuolar membranes via retromer subunit VPS35. Interacts with the HOPS complex subunit VPS39 independent of the HOPS complex at mitochondria-vacuole contact sites (vCLAMPs), providing a physical and metabolic interconnection between the endocytic pathway and mitochondria.

Gene References into Functions

phosphorylation of vacuole protein sorting complex (HOPS) by recombinant Yck3p confers strict requirement for GTP-bound Ypt7p for binding phosphorylated HOPS, for optimal membrane tethering and proteoliposome fusion; guanine nucleotide specificity of the vacuolar fusion Rab Ypt7p is conferred through downstream posttranslational modification of its effector complex PMID: 22787280
yeast retromer trimer antagonizes Ypt7-regulated organelle tethering and fusion of endosomes/vacuoles via recognition of Ypt7 PMID: 22593205
msb3 vacuoles fuse more efficiently than wild-type vacuoles in vitro, suggesting that GAP can also act on Ypt7 PMID: 22593206
Ypt7p plays a role beyond homotypic fusion and protein sorting-Class C Vps complex recruitment in membrane fusion PMID: 22417749
Ypt7 functions at the late endosome to coordinate retromer-mediated recycling with the fusion of late endosomes with vacuoles. PMID: 21062894
We also show that expression of a mutated variant of the YPT7 gene suppresses the sporulation and autophagy defects of ccz1Delta cells to a quantitatively similar level. PMID: 20709422
apparent forward rate constant of 4 soluble N-ethylmaleimide-sensitive factor attachment protein receptors/vacuole protein sorting complex complex assembly is enhanced many thousand-fold by Ypt7p PMID: 17699614
although Ypt7p may contribute to other fusion functions, its central role is to bind the homoytpic fusion and vacuole protein sorting complex to the membrane PMID: 19386605

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Subcellular Location

Late endosome. Vacuole membrane.

Protein Families

Small GTPase superfamily, Rab family

Database Links

KEGG: sce:YML001W

STRING: 4932.YML001W

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Recombinant Saccharomyces cerevisiae GTP-binding protein YPT7 (YPT7)

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